[HTML][HTML] Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%

J Goedhart, D Von Stetten, M Noirclerc-Savoye… - Nature …, 2012 - nature.com
… (d) pH dependence of mTurquoise2 fluorescence. Each point corresponds to the average of
three measurements and is represented with the standard deviation. Data … David von Stetten

X-ray screening identifies active site and allosteric inhibitors of SARS-CoV-2 main protease

…, M Wolf, AR Beccari, G Bourenkov, D von Stetten… - Science, 2021 - science.org
The coronavirus disease (COVID-19) caused by SARS-CoV-2 is creating tremendous human
suffering. To date, no effective drug is available to directly treat the disease. In a search for …

[HTML][HTML] Mutational analysis of Deinococcus radiodurans bacteriophytochrome reveals key amino acids necessary for the photochromicity and proton exchange cycle …

JR Wagner, J Zhang, D Von Stetten, M Günther… - Journal of Biological …, 2008 - ASBMB
The ability of phytochromes (Phy) to act as photointerconvertible light switches in plants and
microorganisms depends on key interactions between the bilin chromophore and the …

[HTML][HTML] Light-induced Proton Release of Phytochrome Is Coupled to the Transient Deprotonation of the Tetrapyrrole Chromophore*[boxs]

B Borucki, D von Stetten, S Seibeck, T Lamparter… - Journal of Biological …, 2005 - ASBMB
… David von Stetten David von Stetten … We conclude, therefore, that the changes of the
time constants and amplitudes in D 2 O represent true kinetic isotope effects. …

[HTML][HTML] Crystal structure of a common GPCR-binding interface for G protein and arrestin

…, R Kazmin, A Rose, FJ Bartl, D Von Stetten… - Nature …, 2014 - nature.com
… After regeneration with 30 μM 11-cis-retinal the protein was purified in 1% n-D-octyl-b-D-glucopyranoside
using the 1D4 antibody bound to sepharose resin. Rhodopsin was eluted from …

[HTML][HTML] MASSIF-1: a beamline dedicated to the fully automatic characterization and data collection from crystals of biological macromolecules

…, W Schmidt, A Snigirev, D von Stetten… - Journal of synchrotron …, 2015 - scripts.iucr.org
MASSIF-1 (ID30A-1) is an ESRF undulator beamline operating at a fixed wavelength of
0.969 Å (12.8 keV) that is dedicated to the completely automatic characterization of and data …

The status of the macromolecular crystallography beamlines at the European Synchrotron Radiation Facility

…, A Royant, D de Sanctis, D von Stetten… - The European Physical …, 2015 - Springer
The European Synchrotron Radiation Facility (ESRF) is the oldest and most powerful 3rd
generation synchrotron in Europe, providing X-rays to more than 40 experimental stations …

[HTML][HTML] Highly conserved residues Asp-197 and His-250 in Agp1 phytochrome control the proton affinity of the chromophore and Pfr formation

D von Stetten, S Seibeck, N Michael, P Scheerer… - Journal of Biological …, 2007 - ASBMB
… David von Stetten David von Stetten … conformation for the A-B and B-C bridges, whereas
the C-D methine bridge single bond is in the anti conformation. Except for the syn conformation …

[HTML][HTML] In crystallo optical spectroscopy (icOS) as a complementary tool on the macromolecular crystallography beamlines of the ESRF

D von Stetten, T Giraud, P Carpentier… - … Section D: Biological …, 2015 - scripts.iucr.org
The analysis of structural data obtained by X-ray crystallography benefits from information
obtained from complementary techniques, especially as applied to the crystals themselves. As …

Liquid application method for time-resolved analyses by serial synchrotron crystallography

…, M Agthe, S Horrell, G Bourenkov, D von Stetten… - Nature …, 2019 - nature.com
… XI catalyzes the reversible isomerization of an aldose, d-(+)xylose into the ketose, d-xylulose
as well as the conversion of d-glucose into d-fructose, which is of great commercial …