Argonaute proteins are conserved throughout all domains of life. Recently characterized
prokaryotic Argonaute proteins (pAgos) participate in host defense by DNA interference, …

All organisms need to continuously adapt to changes in their environment. Through horizontal
gene transfer, bacteria and archaea can rapidly acquire new traits that may contribute to …

Cas9 and Cas12a are multidomain CRISPR‐associated nucleases that can be programmed
with a guide RNA to bind and cleave complementary DNA targets. The guide RNA …

Background CRISPR/Cas is a widespread adaptive immune system in prokaryotes. This
system integrates short stretches of DNA derived from invading nucleic acids into genomic …

The CRISPR-associated protein Cas12a (Cpf1), which has been repurposed for genome
editing, possesses two distinct nuclease activities: endoribonuclease activity for processing its …

RNA interference is widely distributed in eukaryotes and has a variety of functions, including
antiviral defence and gene regulation 1 , 2 . All RNA interference pathways use small single-…

CRISPR-Cas12a (Cpf1) is an RNA-guided DNA-cutting nuclease that has been repurposed
for genome editing. Upon target DNA binding, Cas12a cleaves both the target DNA in cis …

… 0 -P binding residues in the pAgo MID domain (Figure S1C), we hypothesized that in … of the
Swarts lab and the Laboratory of Biochemistry for discussion. Research in the Swarts lab was …

Functions of prokaryotic Argonautes (pAgo) have long remained elusive. Recently, Argonautes
of the bacteria Rhodobacter sphaeroides and Thermus thermophilus were demonstrated …

We report on crystal structures of ternary Thermus thermophilus Argonaute (TtAgo) complexes
with 5′-phosphorylated guide DNA and a series of DNA targets. These ternary complex …