Mutations in whole organisms are powerful ways of interrogating gene function in a realistic
context. We describe a program, the Sanger Institute Mouse Genetics Project, that provides …

The DNA-binding protein PRDM9 directs positioning of the double-strand breaks (DSBs)
that initiate meiotic recombination in mice and humans. Prdm9 is the only mammalian …

The genome is organized via CTCF–cohesin-binding sites, which partition chromosomes into
1–5 megabase (Mb) topologically associated domains (TADs), and further into smaller sub-…

Iron is essential to the cell. Both iron deficiency and overload impinge negatively on cardiac
health. Thus, effective iron homeostasis is important for cardiac function. Ferroportin (FPN), …

10.7554/eLife.19804.001 Hepcidin is the master regulator of systemic iron homeostasis.
Derived primarily from the liver, it inhibits the iron exporter ferroportin in the gut and spleen, the …

Background Multiple attempts at tracheal intubation are associated with mortality, and
successful rescue requires a structured plan. However, there remains a paucity of data to guide …

Angiogenesis, the fundamental process by which new blood vessels form from existing ones,
depends on precise spatial and temporal gene expression within specific compartments of …

Background A Hill-Sachs lesion of the humeral head after a shoulder dislocation is clinically
insignificant in most cases. However, a sizable defect will engage with the anterior rim of the …

Mutations in the gene encoding the RNA-binding protein TDP-43 cause amyotrophic lateral
sclerosis (ALS), clinically and pathologically indistinguishable from the majority of ‘sporadic’ …

CRISPR/Cas9 machinery delivered as ribonucleoprotein (RNP) to the zygote has become a
standard tool for the development of genetically modified mouse models. In recent years, a …