… Daniel L. Hartl …

A method is presented for the rapid in vitro amplification of DNA sequences that flank a region
of known sequence. The method uses the polymerase chain reaction (PCR), but it has the …

Five point mutations in a particular β-lactamase allele jointly increase bacterial resistance to
a clinically important antibiotic by a factor of ∼100,000. In principle, evolution to this high-…

Nuclear copies of mitochondrial DNA (mtDNA) have contaminated PCR-based mitochondrial
studies of over 64 different animal species. Since the last review of these nuclear …

The overall theme of this volume, which is divided into 4 chapters, is the origin, maintenance
and significance of genetic variation. Chapter 1 focuses on detection and measurement of …

The mutation process ultimately defines the genetic features of all populations and, hence,
has a bearing on a wide range of issues involving evolutionary genetics, inheritance, and …

Antibiotic resistance can evolve through the sequential accumulation of multiple mutations 1
. To study such gradual evolution, we developed a selection device, the 'morbidostat', that …

… Conner and Daniel L. … Conner and Hartl then guide the reader through theoretical and
empirical advances gained from two complementary approaches to this field. The first is the …

Proteins are finicky molecules; they are barely stable and are prone to aggregate, but they
must function in a crowded environment that is full of degradative enzymes bent on their …

Comparison of the gene-expression profiles between adults of Drosophila melanogaster
and Drosophila simulans has uncovered the evolution of genes that exhibit sex-dependent …