The minute photosynthetic prokaryote Prochlorococcus, which was discovered about 10
years ago, has proven exceptional from several standpoints. Its tiny size (0.5 to 0.7 μm in …

The interrogation of genetic markers in environmental meta-barcoding studies is currently
seriously hindered by the lack of taxonomically curated reference data sets for the targeted …

The novel dye SYBR Green I binds specifically to nucleic acids and can be excited by blue
light (488-nm wavelength). Cell concentrations of prokaryotes measured in marine samples …

Picoplankton—cells with a diameter of less than 3 µm—are the dominant contributors to both
primary production and biomass in open oceanic regions 1 , 2 . However, compared with …

Current sampling of genomic sequence data from eukaryotes is relatively poor, biased, and
inadequate to address important questions about their biology, evolution, and ecology; this …

Flow cytometry (FCM) was successfully used to enumerate viruses in seawater after staining
with the nucleic acid-specific dye SYBR Green-I. The technique was first optimized by using …

Assessments of plankton community structure in the oligotrophic oceans based solely on
microscopy may overstate the importance of heterotrophic bacterial biomass. Using flow …

Photosynthetic prokaryotes evolving oxygen are a major component of oceanic ecosystems.
The diversity ofthese micro-organisms in the picoplanktonie fraction of open oceans …

A quantitative PCR (QPCR) assay based on the use of SYBR Green I was developed to
assess the abundance of specific groups of picoeukaryotes in marine waters. Six primer sets …

Symbioses between nitrogen (N) 2 –fixing prokaryotes and photosynthetic eukaryotes are
important for nitrogen acquisition in N-limited environments. Recently, a widely distributed …