Although catalytic mechanisms in natural enzymes are well understood, achieving the diverse
palette of reaction chemistries in re-engineered native proteins has proved challenging. …

The successful use of man-made proteins to advance synthetic biology requires both the
fabrication of functional artificial proteins in a living environment, and the ability of these …

The transport of proteins across or into membranes is a vital biological process, achieved in
every cell by the conserved Sec machinery. In bacteria, SecYEG combines with the SecA …

Protein translocation is a fundamental process in biology. Major gaps in our understanding
of this process arise due the poor sensitivity, low time resolution and irreproducibility of …

The universally conserved Sec system is the primary method cells utilize to transport proteins
across membranes. Until recently, measuring the activity—a prerequisite for understanding …

The outer-membrane of Gram-negative bacteria is critical for surface adhesion, pathogenicity,
antibiotic resistance and survival. The major constituent – hydrophobic β-barrel Outer-…

The Sec translocon is a highly conserved membrane assembly for polypeptide transport
across, or into, lipid bilayers. In bacteria, secretion through the core channel complex—SecYEG …

Fluorescent proteins (FPs) are commonly used in pairs to monitor dynamic biomolecular
events through changes in proximity via distance dependent processes such as Förster …

Construction of artificial higher order protein complexes allows sampling of structural
architectures and functional features not accessible by classical monomeric proteins. Here, we …

Central to the design of an efficient de novo enzyme is a robust yet mutable protein scaffold.
The maquette approach to protein design offers precisely this, employing simple four-α-helix …