The discovery that RNA interference (RNAi) is functional in mammalian cells led us to form
The RNAi Consortium (TRC) with the goal of enabling large-scale loss-of-function screens …

The simplicity of programming the CRISPR (clustered regularly interspaced short palindromic
repeats)–associated nuclease Cas9 to modify specific genomic loci suggests a new way to …

The proto-oncogene KRAS is mutated in a wide array of human cancers, most of which are
aggressive and respond poorly to standard therapies. Although the identification of specific …

We previously piloted the concept of a Connectivity Map (CMap), whereby genes, drugs, and
disease states are connected by virtue of common gene-expression signatures. Here, we …

CRISPR-Cas9–based genetic screens are a powerful new tool in biology. By simply altering
the sequence of the single-guide RNA (sgRNA), one can reprogram Cas9 to target different …

To enable arrayed or pooled loss-of-function screens in a wide range of mammalian cell
types, including primary and nondividing cells, we are developing lentiviral short hairpin RNA (…

Oncogenic mutations in the serine/threonine kinase B-RAF (also known as BRAF) are found
in 50–70% of malignant melanomas 1 . Pre-clinical studies have demonstrated that the B-…

CRISPR-based approaches have quickly become a favored method to perturb genes to
uncover their functions. Here, we review the key considerations in the design of genome editing …

Cancer cells adapt their metabolic processes to drive macromolecular biosynthesis for rapid
cell growth and proliferation 1 , 2 . RNA interference (RNAi)-based loss-of-function …

… assess the FDR level of E ij . Specifically, to assess the FDR for each enrichment value E ij ,
we … of values greater than E ij as well as a vector of values greater than E ij corresponding to …