Cell-free gene expression (CFE) emerged as an alternative approach to living cells for
specific applications in protein synthesis and labelling for structural biology and proteomics …

Highlights • Cell-free expression is a highly multidisciplinary bioengineering technology. •
Cell-free expression is fast, quantitative and a safe synthetic biology environment. • TXTL …

The new generation of cell-free gene expression systems enables the prototyping and
engineering of biological systems in vitro over a remarkable scope of applications and physical …

Rationale Cell‐free transcription‐translation (TXTL) is becoming a popular technology to
prototype and engineer biological systems outside living organisms. TXTL relies commonly on …

Encapsulating biological materials in lipid vesicles is of interest for mimicking cells; however,
except in some particular cases, such processes do not occur spontaneously. Herein, we …

Cell‐free expression systems enable rapid prototyping of genetic programs in vitro. However,
current throughput of cell‐free measurements is limited by the use of channel‐limited …

Executing gene circuits by cell-free transcription−translation into cell-sized compartments,
such as liposomes, is one of the major bottom-up approaches to building minimal cells. The …

The efficacy of cell-free gene expression (CFE) has been considerably improved in the last
decade. As a consequence, CFE systems have now the capacity to express DNAs composed …

The assembly of protein machines in cells is precise, rapid, and coupled to protein synthesis
with regulation in space and time. The assembly of natural and synthetic nanomachines …

Liquid–liquid phase separation (LLPS) is important to control a wide range of reactions from
gene expression to protein degradation in a cell-sized space. To bring a better …