In spite of its recent achievements, the technique of single particle electron cryomicroscopy (cryoEM)
has not been widely used to study proteins smaller than 100 kDa, although it is a …

Single particle electron microscopy (EM), of both negative stained or frozen hydrated biological
samples, has become a versatile tool in structural biology 1. In recent years, this method …

Diverse microbial ecosystems underpin life in the sea. Among these microbes are many
unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic …

RNA is a crucial structural component of many ribonucleoprotein (RNP) complexes, including
the ribosome, spliceosome, and signal recognition particle, but the role of RNA in guiding …

10.7554/eLife.04121.001 The HIV Rev protein routes viral RNAs containing the Rev Response
Element (RRE) through the Crm1 nuclear export pathway to the cytoplasm where viral …

As the closest living relatives of animals, choanoflagellates offer unique insights into animal
origins and core mechanisms underlying animal cell biology. However, unlike traditional …

In a previous study, we established a forward genetic screen to identify genes required for
multicellular development in the choanoflagellate, Salpingoeca rosetta (Levin et al., 2014). Yet…

The signal recognition particle (SRP) recognizes polypeptide chains bearing a signal sequence
as they emerge from the ribosome, and then binds its membrane-associated receptor (…

Transcription factors regulate eukaryotic RNA polymerase II (Pol II) activity by assembling
and remodeling complexes at multiple steps in the transcription cycle. In HIV, we previously …

10.7554/eLife.41482.001 In a previous study we established forward genetics in the
choanoflagellate Salpingoeca rosetta and found that a C-type lectin gene is required for rosette …