Recent advances in next-generation sequencing technology allow high-throughput cDNA
sequencing (RNA-Seq) to be widely applied in transcriptomic studies, in particular for …

A three-dimensional chromatin state underpins the structural and functional basis of the
genome by bringing regulatory elements and genes into close spatial proximity to ensure proper…

Double-stranded DNA is able to form triple-helical structures by accommodating a third
nucleotide strand in its major groove. This sequence-specific process offers a potent mechanism …

Motivation: Transcription factors (TFs) are crucial during the lifetime of the cell. Their functional
roles are defined by the genes they regulate. Uncovering these roles not only sheds light …

Recent years have seen the development of computational tools to assist researchers in
performing CRISPR-Cas9 experiment optimally. More specifically, these tools aim to maximize …

Precise genomic modification using prime editing (PE) holds enormous potential for
research and clinical applications. In this study, we generated all-in-one prime editing (PEA1) …

The capacity of our gut microbial communities to maintain a stable and balanced state,
termed ‘resilience’, in spite of perturbations is vital to our achieving and maintaining optimal …

The benefits of implementing high throughput sequencing in the clinic are quickly becoming
apparent. However, few freely available bioinformatics pipelines have been built from the …

Motivation: Bioinformatics tools, such as assemblers and aligners, are expected to produce
more accurate results when given better quality sequence data as their starting point. This …

Background Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease with
phenotypic and genetic heterogeneity. Approximately 10% of cases are familial, while …