The erbB/HER family of transmembrane receptor tyrosine kinases (RTKs) mediate cellular
responses to epidermal growth factor (EGF) and related ligands. We have imaged the early …

The actin cytoskeleton has been implicated in restricting diffusion of plasma membrane
components. Here, simultaneous observations of quantum dot-labelled FcɛRI motion and GFP-…

Epidermal growth factor receptor (EGFR) regulates many crucial cellular programs, with seven
different activating ligands shaping cell signaling in distinct ways. Using crystallography …

The extent to which ligand occupancy and dimerization contribute to erbB1 signaling is
controversial. To examine this, we used two-color quantum-dot tracking for visualization of the …

ErbB1 receptors situated on cellular filopodia undergo systematic retrograde transport after
binding of the epidermal growth factor (EGF) and activation of the receptor tyrosine kinase. …

Phagocytic receptors must diffuse laterally to become activated upon clustering by multivalent
targets. Receptor diffusion, however, can be obstructed by transmembrane proteins ("…

A fundamental goal in biology is to determine how cellular organization is coupled to function.
To achieve this goal, a better understanding of organelle composition and structure is …

Crosslinking of IgE-bound FcɛRI triggers mast cell degranulation. Previous fluorescence
recovery after photobleaching (FRAP) and phosphorescent anisotropy studies suggested that …

We report a method for tracking individual quantum dot (QD) labeled proteins inside of live
cells that uses four overlapping confocal volume elements and active feedback once every 5 …

Upon activation, ERKs translocate from the cytoplasm to the nucleus. This process is required
for the induction of many cellular responses, yet the molecular mechanisms that regulate …