Although Cas9-mediated genome editing has proven to be a powerful genetic tool in
eukaryotes, its application in Bacteria has been limited because of inefficient targeting or repair; …

10.7554/eLife.29218.001 Methyl-coenzyme M reductase (MCR), found in strictly anaerobic
methanogenic and methanotrophic archaea, catalyzes the reversible production and …

The enzyme methyl-coenzyme M reductase (MCR) plays an important role in mediating
global levels of methane by catalyzing a reversible reaction that leads to the production or …

Normal cellular processes give rise to toxic metabolites that cells must mitigate. Formaldehyde
is a universal stressor and potent metabolic toxin that is generated in organisms from …

Methylotrophs grow on reduced single-carbon compounds like methylamine as the sole
source of carbon and energy. In Methylobacterium extorquens AM1, the best-studied aerobic …

Methanogenic archaea belonging to the Order Methanosarcinales conserve energy using
an electron transport chain (ETC). In the genetically tractable strain Methanosarcina …

Methylobacterium extorquens AM1, a strain serendipitously isolated half a century ago, has
become the best-characterized model system for the study of aerobic methylotrophy (the …

Methyl-coenzyme M reductase (MCR) catalyzes the formation of methane, and its activity
accounts for nearly all biologically produced methane released into the atmosphere. The …

… The authors would like to acknowledge members of the Nayak lab for their feedback and
input. Molecular graphics were produced using the UCSF Chimera package from the …

Microorganisms often encode multiple non-orthologous metabolic modules that catalyze the
same reaction. However, little experimental evidence actually demonstrates a selective …