Signal transduction pathways are modular composites of functionally interdependent sets of
proteins that act in a coordinated fashion to transform environmental information into a …

We describe a chemical proteomics approach to profile the interaction of small molecules
with hundreds of endogenously expressed protein kinases and purine-binding proteins. This …

INTRODUCTION Understanding drug mechanism poses the daunting challenge of determining
the affinity of the drug for all potential targets. Drug target engagement can be assessed …

The jumonji (JMJ) family of histone demethylases are Fe 2+ - and α-ketoglutarate-dependent
oxygenases that are essential components of regulatory transcriptional chromatin …

The development of selective histone deacetylase (HDAC) inhibitors with anti-cancer and
anti-inflammatory properties remains challenging in large part owing to the difficulty of probing …

PAD4 has been strongly implicated in the pathogenesis of autoimmune, cardiovascular and
oncological diseases through clinical genetics and gene disruption in mice. New selective …

We extended thermal proteome profiling to detect transmembrane protein–small molecule
interactions in cultured human cells. When we assessed the effects of detergents on ATP-…

Isobaric stable isotope tagging reagents such as tandem mass tags or isobaric tags for relative
and absolute quantification enable multiplexed quantification of peptides via reporter ion …

Pax5 (BSAP) functions as both a transcriptional activator and repressor during midbrain
patterning, B‐cell development and lymphomagenesis. Here we demonstrate that Pax5 exerts …

Gene knock outs (KOs) are efficiently engineered through CRISPR–Cas9-induced frameshift
mutations. While the efficiency of DNA editing is readily verified by DNA sequencing, a …