Alternative splicing and nonsense-mediated decay modulate expression of important regulatory genes in Arabidopsis

…, CG Simpson, NH Syed, D Lewandowska… - Nucleic acids …, 2012 - academic.oup.com
Alternative splicing (AS) coupled to nonsense-mediated decay (NMD) is a post-transcriptional
mechanism for regulating gene expression. We have used a high-resolution AS RT–PCR …

Proteomic Analysis of the Arabidopsis Nucleolus Suggests Novel Nucleolar Functions

…, GP Clark, R Boon, D Lewandowska… - Molecular biology of …, 2005 - Am Soc Cell Biol
The eukaryotic nucleolus is involved in ribosome biogenesis and a wide range of other
RNA metabolism and cellular functions. An important step in the functional analysis of the …

A high quality Arabidopsis transcriptome for accurate transcript-level analysis of alternative splicing

…, M Spensley, JC Entizne, D Lewandowska… - Nucleic acids …, 2017 - academic.oup.com
Alternative splicing generates multiple transcript and protein isoforms from the same gene
and thus is important in gene expression regulation. To date, RNA-sequencing (RNA-seq) is …

Involvement of the nuclear cap-binding protein complex in alternative splicing in Arabidopsis thaliana

…, A Ciesiolka, L Szewc, D Lewandowska… - Nucleic Acids …, 2010 - academic.oup.com
The nuclear cap-binding protein complex (CBC) participates in 5′ splice site selection of
introns that are proximal to the mRNA cap. However, it is not known whether CBC has a role in …

Aberrant mRNA Transcripts and the Nonsense-Mediated Decay Proteins UPF2 and UPF3 Are Enriched in the Arabidopsis Nucleolus

SH Kim, OA Koroleva, D Lewandowska… - The Plant …, 2009 - academic.oup.com
The eukaryotic nucleolus is multifunctional and involved in the metabolism and assembly of
many different RNAs and ribonucleoprotein particles as well as in cellular functions, such as …

[HTML][HTML] Identification and rapid mapping of a gene conferring broad-spectrum late blight resistance in the diploid potato species Solanum verrucosum through DNA …

X Chen, D Lewandowska, MR Armstrong… - Theoretical and Applied …, 2018 - Springer
Key message A broad-spectrum late blight disease-resistance gene from Solanum verrucosum
has been mapped to potato chromosome 9. The gene is distinct from previously identified…

[HTML][HTML] Plant SILAC: Stable-Isotope Labelling with Amino Acids of Arabidopsis Seedlings for Quantitative Proteomics

D Lewandowska, S ten Have, K Hodge, V Tillemans… - PLoS …, 2013 - journals.plos.org
Stable Isotope Labelling by Amino acids in Cell culture (SILAC) is a powerful technique for
comparative quantitative proteomics, which has recently been applied to a number of different …

[HTML][HTML] Barley anther and meiocyte transcriptome dynamics in meiotic prophase I

…, J Orr, M Schreiber, I Colas, D Lewandowska… - Frontiers in plant …, 2021 - frontiersin.org
In flowering plants, successful germinal cell development and meiotic recombination
depend upon a combination of environmental and genetic factors. To gain insights into this …

Cloning and characterization of two subunits of Arabidopsis thaliana nuclear cap-binding complex

M Kmieciak, CG Simpson, D Lewandowska… - Gene, 2002 - Elsevier
In this report we characterize two Arabidopsisthaliana proteins, named AtCBP20 and AtCBP80,
that are homologues of human subunits of a nuclear cap-binding protein complex (CBC). …

Determinants of plant U12-dependent intron splicing efficiency

D Lewandowska, CG Simpson, GP Clark… - The Plant …, 2004 - academic.oup.com
Factors affecting splicing of plant U12-dependent introns have been examined by extensive
mutational analyses in an in vivo tobacco (Nicotiana tabacum) protoplast system using …