User profiles for Dustin R. Klein

Dustin R. Klein

Senior Scientist, Merck
Verified email at merck.com
Cited by 1256

Pinpointing Double Bond and sn-Positions in Glycerophospholipids via Hybrid 193 nm Ultraviolet Photodissociation (UVPD) Mass Spectrometry

PE Williams, DR Klein, SM Greer… - Journal of the American …, 2017 - ACS Publications
Complete structural characterization of complex lipids, such as glycerophospholipids, by
tandem mass spectrometry (MS/MS) continues to present a major challenge. Conventional …

Reinforcing lipid A acylation on the cell surface of Acinetobacter baumannii promotes cationic antimicrobial peptide resistance and desiccation survival

JM Boll, AT Tucker, DR Klein, AM Beltran, JS Brodbelt… - MBio, 2015 - Am Soc Microbiol
Acinetobacter baumannii is an emerging Gram-negative pathogen found in hospitals and
intensive care units. In order to persist in hospital environments, A. baumannii withstands …

Structural characterization of phosphatidylcholines using 193 nm ultraviolet photodissociation mass spectrometry

DR Klein, JS Brodbelt - Analytical chemistry, 2017 - ACS Publications
Advances in mass spectrometry have made it a preferred tool for structural characterization
of glycerophospholipids. Collisional activation methods commonly implemented on …

Desorption electrospray ionization mass spectrometry imaging of proteins directly from biological tissue sections

KY Garza, CL Feider, DR Klein, JA Rosenberg… - Analytical …, 2018 - ACS Publications
Analysis of large biomolecules including proteins has proven challenging using ambient
ionization mass spectrometry imaging techniques. Here, we have successfully optimized …

Resolving the complexity of spatial lipidomics using MALDI TIMS imaging mass spectrometry

KV Djambazova, DR Klein, LG Migas… - Analytical …, 2020 - ACS Publications
Lipids are a structurally diverse class of molecules with important biological functions
including cellular signaling and energy storage. Matrix-assisted laser desorption/ionization (MALDI…

Shotgun analysis of rough-type lipopolysaccharides using ultraviolet photodissociation mass spectrometry

DR Klein, DD Holden, JS Brodbelt - Analytical chemistry, 2016 - ACS Publications
Detailed structural characterization of intact rough-type lipopolysaccharides (R-LPS) was
accomplished using a multi-stage mass spectrometry (MS 3 ) strategy consisting of collision-…

Desorption electrospray ionization coupled with ultraviolet photodissociation for characterization of phospholipid isomers in tissue sections

DR Klein, CL Feider, KY Garza, JQ Lin… - Analytical …, 2018 - ACS Publications
Desorption electrospray ionization (DESI) mass spectrometry imaging has become a powerful
strategy for analysis of tissue sections, enabling differentiation of normal and diseased …

Colistin heteroresistance in Enterobacter cloacae is regulated by PhoPQ‐dependent 4‐amino‐4‐deoxy‐l‐arabinose addition to lipid A

KN Kang, DR Klein, MI Kazi, F Guérin… - Molecular …, 2019 - Wiley Online Library
The Enterobacter cloacae complex (ECC) consists of closely related bacteria commonly
associated with the human microbiota. ECC are increasingly isolated from healthcare‐…

Localization of cyclopropane modifications in bacterial lipids via 213 nm ultraviolet photodissociation mass spectrometry

MS Blevins, DR Klein, JS Brodbelt - Analytical chemistry, 2019 - ACS Publications
Subtle structural features in bacterial lipids such as unsaturation elements can have vast
biological implications. Cyclopropane rings have been correlated with tolerance to a number of …

Effect of MALDI matrices on lipid analyses of biological tissues using MALDI‐2 postionization mass spectrometry

JC McMillen, JA Fincher, DR Klein… - Journal of mass …, 2020 - Wiley Online Library
Matrix‐assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) allows
for highly multiplexed, untargeted detection of many hundreds of analytes from tissue. …