User profiles for Dylan M Owen
 | Dylan M OwenUniversity of Birmingham Verified email at bham.ac.uk Cited by 6857 |
[HTML][HTML] Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations
Despite significant progress, high-speed live-cell super-resolution studies remain limited to
specialized optical setups, generally requiring intense phototoxic illumination. Here, we …
specialized optical setups, generally requiring intense phototoxic illumination. Here, we …
Quantitative imaging of membrane lipid order in cells and organisms
It is now recognized that lipids and proteins in cellular membranes are not homogenously
distributed. A high degree of membrane order is the biophysical hallmark of cholesterol-…
distributed. A high degree of membrane order is the biophysical hallmark of cholesterol-…
Pre-existing clusters of the adaptor Lat do not participate in early T cell signaling events
Engaged T cell antigen receptors (TCRs) initiate signaling through the adaptor protein Lat.
In quiescent T cells, Lat is segregated into clusters on the cell surface, which raises the …
In quiescent T cells, Lat is segregated into clusters on the cell surface, which raises the …
PALM imaging and cluster analysis of protein heterogeneity at the cell surface
The authors employed photoactivatable localization microscopy (PALM) and direct stochastic
optical reconstruction microscopy (dSTORM) imaging and image analysis based on …
optical reconstruction microscopy (dSTORM) imaging and image analysis based on …
[HTML][HTML] Sub-resolution lipid domains exist in the plasma membrane and regulate protein diffusion and distribution
DM Owen, DJ Williamson, A Magenau… - Nature communications, 2012 - nature.com
Lipid microdomains are postulated to regulate many membrane-associated processes but
have remained highly controversial. Here we provide the first direct evidence that the plasma …
have remained highly controversial. Here we provide the first direct evidence that the plasma …
Conformational states of the kinase Lck regulate clustering in early T cell signaling
Phosphorylation of the T cell antigen receptor (TCR) by the tyrosine kinase Lck is an essential
step in the activation of T cells. Because Lck is constitutively active, spatial organization …
step in the activation of T cells. Because Lck is constitutively active, spatial organization …
The lipid raft hypothesis revisited–New insights on raft composition and function from super‐resolution fluorescence microscopy
Recently developed super‐resolution microscopy techniques are changing our understanding
of lipid rafts and membrane organisation in general. The lipid raft hypothesis postulates …
of lipid rafts and membrane organisation in general. The lipid raft hypothesis postulates …
Differential effects of lipids and lyso-lipids on the mechanosensitivity of the mechanosensitive channels MscL and MscS
Mechanosensitive (MS) channels of small (MscS) and large (MscL) conductance are the
major players in the protection of bacterial cells against hypoosmotic shock. Although a great …
major players in the protection of bacterial cells against hypoosmotic shock. Although a great …
Turning single-molecule localization microscopy into a quantitative bioanalytical tool
Single-molecule localization microscopy (SMLM) generates super-resolution images by
serially detecting individual fluorescent molecules. The power of SMLM, however, goes beyond …
serially detecting individual fluorescent molecules. The power of SMLM, however, goes beyond …
[PDF][PDF] Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells
DM Owen, PMP Lanigan, C Dunsby, I Munro, D Grant… - Biophysical journal, 2006 - cell.com
We apply fluorescence lifetime imaging to the membrane phase-sensing dye di-4-ANEPPDHQ
in model membranes and live cells. We show that the 1700ps lifetime shift between liquid-…
in model membranes and live cells. We show that the 1700ps lifetime shift between liquid-…