Electron microscopy remains one of the most accurate methods for the detection of autophagy
and quantification of autophagic accumulation. Compared to fluorescence microscopy, …

Autophagosomes have been reported to form in the vicinity of the endoplasmic reticulum (ER).
In many cases, the phagophore membrane is observed between two cisternae of rough …

Transplantation of pancreatic islet cells derived from human pluripotent stem cells is a promising
treatment for diabetes. Despite progress in the generation of stem-cell-derived islets (SC…

Modern research in the life sciences is unthinkable without computational methods for
extracting, quantifying and visualising information derived from microscopy imaging data of …

… All of the cals3-d had shorter roots than wild-type (Figure 1E); we considered cals3-2d as
the most severe allele as it had the shortest root (Figure 1E). In heterozygous cals3-d mutants …

The actin cytoskeleton plays a fundamental role in various motile and morphogenetic
processes involving membrane dynamics. We show that actin-binding proteins MIM (missing-in-…

The organization of multiple mitochondrial DNA (mtDNA) molecules in discrete protein-DNA
complexes called nucleoids is well studied inSaccharomyces cerevisiae. Similar structures …

… (G) Representative E-cadherin (E-cad) images and quantification show time-dependent
reorientation of E-cad-positive adherens junctions 45 away from 40% stretch direction (…

Seipin is an endoplasmic reticulum ( ER ) membrane protein implicated in lipid droplet ( LD
) biogenesis and mutated in severe congenital lipodystrophy ( BSCL 2). Here, we show that …

… (E) Stepwise segmentation workflow is needed to generate a 3-D model of a complex
structure. A metaphase Huh-7 cell transiently expressing ssHRP-KDEL was cytochemically …