Organism-level systems biology aims to identify, analyze, control and design cellular circuits
in organisms. Many experimental and computational approaches have been developed …

Systems-level identification and analysis of cellular circuits in the brain will require the
development of whole-brain imaging with single-cell resolution. To this end, we performed …

Here we describe a protocol for advanced CUBIC (Clear, Unobstructed Brain/Body Imaging
Cocktails and Computational analysis). The CUBIC protocol enables simple and efficient …

The development of whole-body imaging at single-cell resolution enables system-level
approaches to studying cellular circuits in organisms. Previous clearing methods focused on …

We describe a strategy for developing hydrophilic chemical cocktails for tissue delipidation,
decoloring, refractive index (RI) matching, and decalcification, based on comprehensive …

Whole-organ/body three-dimensional (3D) staining and imaging have been enduring
challenges in histology. By dissecting the complex physicochemical environment of the staining …

Atherosclerosis is a chronic inflammatory disease of the vascular walls related to aging.
Thus far, the roles of cellular senescence and bacterial infection in the pathogenesis of …

Cell senescence plays a key role in age-associated organ dysfunction, but the in vivo
pathogenesis is largely unclear. Here, we generated a p16-Cre ERT2 -tdTomato mouse model to …

The detailed molecular mechanisms underlying the regulation of sleep duration in mammals
are still elusive. To address this challenge, we constructed a simple computational model, …

The examination of hematoxylin and eosin (H&E)-stained tissues on glass slides by
conventional light microscopy is the foundation for histopathological diagnosis. However, this …