Flow cytometry is an essential tool for dissecting the functional complexity of hematopoiesis.
We used single-cell “mass cytometry” to examine healthy human bone marrow, measuring …

New high-dimensional, single-cell technologies offer unprecedented resolution in the analysis
of heterogeneous tissues. However, because these technologies can measure dozens of …

To guide the design of immunotherapy strategies for patients with early stage lung tumors,
we developed a multiscale immune profiling strategy to map the immune landscape of early …

Tissue regeneration is an orchestrated progression of cells from an immature state to a
mature one, conventionally represented as distinctive cell subsets. A continuum of transitional …

Atherosclerosis is driven by multifaceted contributions of the immune system within the
circulation and at vascular focal sites. However, specific characteristics of dysregulated immune …

Mass-tag cell barcoding (MCB) labels individual cell samples with unique combinatorial
barcodes, after which they are pooled for processing and measurement as a single multiplexed …

Pancreatic ductal adenocarcinoma (PDA) is characterized by an immune-suppressive tumor
microenvironment that renders it largely refractory to immunotherapy. We implemented a …

While conventional pathogenic protists have been extensively studied, there is an
underappreciated constitutive protist microbiota that is an integral part of the vertebrate microbiome. …

Despite recent advances in single-cell genomic, transcriptional, and mass-cytometric profiling,
it remains a challenge to collect highly multiplexed measurements of secreted proteins …

Large-scale immune monitoring experiments (such as clinical trials) are a promising direction
for biomarker discovery and responder stratification in immunotherapy. Mass cytometry is …