Imaging intracellular fluorescent proteins at nanometer resolution

E Betzig, GH Patterson, R Sougrat, OW Lindwasser… - science, 2006 - science.org
… Summing the molecular images across all frames results in a diffraction-limited image (E
′) and summing the results yields a superresolution image (E′ and F′) in which resolution is …

Single Molecules, Cells, and Super-Resolution Optics (Nobel Lecture).

E Betzig - Angewandte Chemie (International ed. in English), 2015 - europepmc.org
Betzig E 1 … In his Nobel Lecture, E. Betzig describes the developments that have led to
modern super high-resolution microscopy. …

Breaking the diffraction barrier: optical microscopy on a nanometric scale

E Betzig, JK Trautman, TD Harris, JS Weiner… - Science, 1991 - science.org
In near-field scanning optical microscopy, a light source or detector with dimensions less than
the wavelength (λ) is placed in close proximity (<λ/50) to a sample to generate images with …

Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution

…, JT Wang, G Seydoux, US Tulu, DP Kiehart, E Betzig - Science, 2014 - science.org
Introduction In vivo imaging provides a window into the spatially complex, rapidly evolving
physiology of the cell that structural imaging alone cannot. However, observing this …

[PDF][PDF] Imaging live-cell dynamics and structure at the single-molecule level

Z Liu, LD Lavis, E Betzig - Molecular cell, 2015 - cell.com
… This requires imaging techniques with different spatial sweet spots, from nanometers to
millimeters, and diverse temporal resolutions, from nanoseconds to days (Figure 2E). This broad …

Single molecules observed by near-field scanning optical microscopy

E Betzig, RJ Chichester - Science, 1993 - science.org
… in its interaction with an incident electromagnetic field, so that each molecule in the field of
view serves as a point detector excited by the square of that component of the electric field E

Near-field optics: microscopy, spectroscopy, and surface modification beyond the diffraction limit

E Betzig, JK Trautman - Science, 1992 - science.org
… and c( NSOM, because they determine ti to which the EM energy in the ne coupled out to
the far-field detecto quently, high-resolution surface inf can be obtained in transmission e thick …

Proposed method for molecular optical imaging

E Betzig - Optics letters, 1995 - opg.optica.org
We can resolve multiple discrete features within a focal region of m spatial dimensions by
first isolating each on the basis of n ≥ 1 unique optical characteristics and then measuring …

Near‐field magneto‐optics and high density data storage

E Betzig, JK Trautman, R Wolfe, EM Gyorgy… - Applied Physics …, 1992 - pubs.aip.org
Near‐field scanning optical microscopy (NSOM) has been used to image and record domains
in thin‐film magneto‐optic (MO) materials. In the imaging mode, resolution of 30–50 nm …

Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination

…, MW Davidson, JA Galbraith, CG Galbraith, E Betzig - Nature …, 2011 - nature.com
A key challenge when imaging living cells is how to noninvasively extract the most
spatiotemporal information possible. Unlike popular wide-field and confocal methods, plane-…