CRISPR/Cas9 Methodology for the Generation of Knockout Deletions in Caenorhabditis elegans
V Au, E Li-Leger, G Raymant, S Flibotte… - G3: Genes …, 2019 - academic.oup.com
The Caenorhabditis elegans Gene Knockout Consortium is tasked with obtaining null mutations
in each of the more than 20,000 open reading frames (ORFs) of this organism. To date, …
in each of the more than 20,000 open reading frames (ORFs) of this organism. To date, …
Metarhizium brunneum–An enzootic wireworm disease and evidence for its suppression by bacterial symbionts
T Kabaluk, E Li-Leger, S Nam - Journal of invertebrate pathology, 2017 - Elsevier
Wireworms (Coleoptera: Elateridae) are serious agricultural pests, with soil-dwelling larvae
attacking subterranean tissues of crop plants and their fruit when in contact with the soil …
attacking subterranean tissues of crop plants and their fruit when in contact with the soil …
Identification of essential genes in Caenorhabditis elegans through whole-genome sequencing of legacy mutant collections
E Li-Leger, R Feichtinger, S Flibotte, H Holzkamp… - G3, 2021 - academic.oup.com
It has been estimated that 15%–30% of the ∼20,000 genes in C. elegans are essential, yet
many of these genes remain to be identified or characterized. With the goal of identifying …
many of these genes remain to be identified or characterized. With the goal of identifying …
[HTML][HTML] Accelerating gene discovery by phenotyping whole-genome sequenced multi-mutation strains and using the sequence kernel association test (SKAT)
…, M Edgley, Q Muncaster, V Au, E Li-Leger… - PLoS …, 2016 - journals.plos.org
… Here we demonstrate that rare-variant association analysis (e.g., SKAT) is an efficient
way to rapidly uncover novel genes for a phenotype of interest (e.g., ciliated sensory neuron …
way to rapidly uncover novel genes for a phenotype of interest (e.g., ciliated sensory neuron …
A Strategy To Isolate Modifiers of Caenorhabditis elegans Lethal Mutations: Investigating the Endoderm Specifying Ability of the Intestinal Differentiation GATA Factor …
The ELT-2 GATA factor normally functions in differentiation of the C. elegans endoderm,
downstream of endoderm specification. We have previously shown that, if ELT-2 is expressed …
downstream of endoderm specification. We have previously shown that, if ELT-2 is expressed …
Optimizing guide RNA selection and CRISPR/Cas9 methodology for efficient generation of deletions in C. elegans
The Caenorhabditis elegans Gene Knockout (KO) Consortium is tasked with obtaining null
mutations in each of the more than 20,000 open reading frames (ORFs) of this organism. To …
mutations in each of the more than 20,000 open reading frames (ORFs) of this organism. To …
Identification of essential genes in C. elegans through whole genome sequencing of legacy mutant collections
E Li-Leger - 2020 - open.library.ubc.ca
It has been estimated that 15-30% of the~ 20,000 genes in C. elegans are essential, yet
many of these genes remain to be identified or characterized. With the goal of identifying …
many of these genes remain to be identified or characterized. With the goal of identifying …
Effect of Copper Sulfate on the Change in Speed of Caenorhabditis elegans
T Chen, E Leonard, E Li-Leger, Y Tomura - The Expedition, 2012 - ojs.library.ubc.ca
Abstract Changes in the behaviour of the free--‐living nematode Caenorhabditis elegans are
believed to be an effective indicator of toxic exposures. Copper is a known neurotoxin of C. …
believed to be an effective indicator of toxic exposures. Copper is a known neurotoxin of C. …
[CITATION][C] CRISPR/Cas9 methodology for the generation of knockout deletions in Caenorhabditis elegans. G3 (Bethesda) 9: 135–144
[CITATION][C] Optimizing guide RNA selection and CRISPR/Cas9 methodology for efficient generation of deletions in C. elegans. bioRxiv 359588
V Au, E Li-Leger, G Raymant, S Flibotte, G Chen - 2018