Background Combining biologics and small molecules could potentially overcome the plateau
of drug efficacy in inflammatory bowel disease (IBD). We conducted a systematic review …

We sought to review the epidemiological features and clinical implications of normal-weight
obesity. The concept of normal-weight obesity has been recently reported as an important …

<p id="p001">Premature ovarian failure is a major cause of female infertility. The genetic
causes of this disorder remain unknown in most patients. Using whole-exome sequence …

… undertook the production of a recombinant form of this enzyme in E. coli. To do that, a 1.5 kb
fragment … The resulting plasmid was then transformed into E. coli BL21(DE3)pLysS, and the …

A new member of the matrix metalloproteinase (MMP) family of enzymes has been cloned
from a human breast carcinoma cDNA library. The isolated cDNA contains on open reading …

… As a preliminary step to elucidate this question, we expressed the cloned cDNA in E. coli.
For this purpose, an 815-bp fragment encoding part of the prodomain and the entire catalytic …

… labeling of BubR1 and CENP-E in almost all the kinetochores … -E, we used a polyclonal
rabbit serum (pAb1.6) that recognizes the neck region (amino acids 256–817) of human CENP-E …

… the pRSETA E. coli expression vector (Invitrogen) previously cleaved with the above
restriction enzymes. The expression vector was transformed into BL21(DE3)pLysS competent E. …

… We express our sincere thanks to Drs E de Boer, T de Lange, R Freire, MA Handel and E
Marcon for providing antibodies and reagents and I Ramos‐Fernández for technical assistance…

Human collagenase-3 (MMP13) is a recently identified member of the matrix metalloproteinase
(MMP) family that is expressed in breast carcinomas and in articular cartilage from …