Metabolomics is the characterization, identification, and quantitation of metabolites resulting
from a wide range of biochemical processes in living systems. Its rapid development over …

Silylation chemistry on porous silicon provides for ultrahigh sensitivity and analyte specificity
with desorption/ionization on silicon mass spectrometry (DIOS-MS) analysis. Here, we …

The analysis of HIV-1 envelope carbohydrates is critical to understanding their roles in HIV-1
transmission as well as in binding of envelope to HIV-1 antibodies. However, direct …

The production of native-like recombinant versions of the HIV-1 envelope glycoprotein (Env)
trimer requires overcoming the natural flexibility and instability of the complex. The …

The human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env) trimer,
which consists of the gp120 and gp41 subunits, is the focus of multiple strategies for vaccine …

The HIV-1 envelope (Env) is a key determinant in mediating viral entry and fusion to host
cells and is a major target for HIV vaccine development. While Env is typically about 50% …

INTRODUCTION A major goal of HIV-1 vaccine development is the design of immunogens
that induce broadly neutralizing antibodies (bnAbs). However, vaccination of humans has not …

Glycosylation plays an essential role in regulating protein function by modulating biological,
structural, and therapeutic properties. However, due to its inherent heterogeneity and …

One of the principal goals of glycoprotein research is to correlate glycan structure and function.
Such correlation is necessary in order for one to understand the mechanisms whereby …

We have investigated factors that influence the production of native-like soluble, recombinant
trimers based on the env genes of two isolates of human immunodeficiency virus type 1 (…