Marked variability of BK virus load measurement using quantitative real-time PCR among commonly used assays
NG Hoffman, L Cook, EE Atienza… - Journal of clinical …, 2008 - Am Soc Microbiol
BK virus (BKV) is the infectious cause of polyomavirus-associated nephropathy. Screening
guidelines for renal-transplant recipients define levels of viremia and viruria that are …
guidelines for renal-transplant recipients define levels of viremia and viruria that are …
Quantification of BK virus standards by quantitative real-time PCR and droplet digital PCR is confounded by multiple virus populations in the WHO BKV international …
AC Bateman, AL Greninger, EE Atienza… - Clinical …, 2017 - academic.oup.com
BACKGROUND The WHO recently released a BK virus (BKV) international standard. This
study evaluated the WHO international standard and commercially available BKV standards …
study evaluated the WHO international standard and commercially available BKV standards …
Detection of Epstein-Barr virus DNA in sera from transplant recipients with lymphoproliferative disorders
AP Limaye, ML Huang, EE Atienza… - Journal of Clinical …, 1999 - Am Soc Microbiol
… Atienza, James M. Ferrenberg, Lawrence Corey… Ederlyn E. Atienza …
Bloodborne viral pathogen contamination in the era of laboratory automation
A Bryan, L Cook, EE Atienza, J Kuypers… - Clinical …, 2016 - academic.oup.com
BACKGROUND The CDC states that laboratory testing for persons under investigation for
Ebola virus disease can be safely performed using automated laboratory instruments by …
Ebola virus disease can be safely performed using automated laboratory instruments by …
Copy number heterogeneity of JC virus standards
AL Greninger, AC Bateman, EE Atienza… - Journal of Clinical …, 2017 - Am Soc Microbiol
Quantitative PCR is a diagnostic mainstay of clinical virology, and accurate quantitation of
viral load among labs requires the use of international standards. However, the use of multiple …
viral load among labs requires the use of international standards. However, the use of multiple …
Comparison of enzyme immunoassays for detection of antibodies to hepatitis D virus in serum
SK Chow, EE Atienza, L Cook, H Prince… - Clinical and Vaccine …, 2016 - Am Soc Microbiol
Serology remains critical for diagnosing hepatitis D virus (HDV) infection, which affects 15 to
20 million people worldwide, but the literature on characterizing commercial enzyme …
20 million people worldwide, but the literature on characterizing commercial enzyme …
Comparison of methods for extraction of viral DNA from cellular specimens
L Cook, EE Atienza, A Bagabag… - … and infectious disease, 2009 - Elsevier
The accuracy and precision of quantitative polymerase chain reaction (PCR) results depend
not only on the PCR reaction but also on the extraction of viral nucleic acid. Although the …
not only on the PCR reaction but also on the extraction of viral nucleic acid. Although the …
Evaluation and utilization of preassembled frozen commercial fast real‐time qPCR master mixes for detection of cytomegalovirus and BK virus
WA Glover, EE Atienza, S Nesbitt… - Journal of Medical …, 2016 - Wiley Online Library
Quantitative DNA detection of cytomegalovirus (CMV) and BK virus (BKV) is critical in the
management of transplant patients. Quantitative laboratory‐developed procedures for CMV …
management of transplant patients. Quantitative laboratory‐developed procedures for CMV …
Standard Issue: Copy number heterogeneity of JC virus standards discovered through next-generation sequencing
AL Greninger, AC Bateman, EE Atienza, S Wendt… - bioRxiv, 2016 - biorxiv.org
Quantitative PCR is a diagnostic mainstay of clinical virology, and accurate quantitation of
viral load among labs requires the use of international standards. However, the use of multiple …
viral load among labs requires the use of international standards. However, the use of multiple …
Calibration curves for real-time PCR
KKY Lai, L Cook, EM Krantz, L Corey… - Clinical …, 2005 - academic.oup.com
Background: Despite the increasing use of real-time PCR in the diagnosis and management
of viral infections, there are no published studies adequately addressing the optimum …
of viral infections, there are no published studies adequately addressing the optimum …