Abstract Analysis of single-cell RNA-seq data begins with pre-processing of sequencing
reads to generate count matrices. We investigate algorithm choices for the challenges of pre-…

The poseidon syringe pump and microscope system is an open source alternative to
commercial systems. It costs less than $400 and can be assembled in under an hour using the …

The construction of physical three-dimensional (3D) models of biomolecules can uniquely
contribute to the study of the structure-function relationship. 3D structures are most often …

This article presents a combination of support vector machine (SVM) and k-nearest neighbor
(k-NN) to monitor rotational machines using vibrational data. The system is used as triage …

To the Editor—Methods for analyzing single-cell data 1, 2, 3, 4 perform a core set of
computational tasks. These tasks include dimensionality reduction, cell clustering, cell-state …

We describe a workflow for preprocessing of single-cell RNA-sequencing data that balances
efficiency and accuracy. Our workflow is based on the kallisto and bustools programs, and …

WormBase ( www.wormbase.org ) is the central repository for the genetics and genomics of
the nematode Caenorhabditis elegans. We provide the research community with data and …

The more than 1000 single-cell transcriptomics studies that have been published to date
constitute a valuable and vast resource for biological discovery. While various ‘atlas’ projects …

This Article contains an error in Figure 4, where the replotting of a subset of data in Figure
4a, which pertain to the Harvard dataset is incorrect in panels (1) and (3). The correct Figure …

The allocation of a sequencing budget when designing single cell RNA-seq experiments
requires consideration of the tradeoff between number of cells sequenced and the read depth …