The ability to visualize biomolecules within living specimen by engineered fluorescence
tags has become a major tool in modern biotechnology and cell biology. Encoding fusion …

Membrane traffic in eukaryotic cells involves transport of vesicles that bud from a donor
compartment and fuse with an acceptor compartment. Common principles of budding and fusion …

Single-molecule Förster resonance energy transfer (smFRET) is increasingly being used to
determine distances, structures, and dynamics of biomolecules in vitro and in vivo. However, …

The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can
be implemented flexibly in living cells and in vivo. However, the design of synthetic …

We studied the coupled binding and folding of α-synuclein, an intrinsically disordered protein
linked with Parkinson's disease. Using single-molecule fluorescence resonance energy …

Nuclear pore complexes are fundamental components of all eukaryotic cells that mediate
nucleocytoplasmic exchange. Determining their 110-megadalton structure imposes a …

The mechanisms by which intrinsically disordered proteins engage in rapid and highly
selective binding is a subject of considerable interest and represents a central paradigm to …

We genetically encoded the photocaged amino acid 4,5-dimethoxy-2-nitrobenzylserine (DMNB-Ser,
1 ) in Saccharomyces cerevisiae in response to the amber nonsense codon TAG. …

The HIV-1 envelope glycoprotein (Env) trimer mediates cell entry and is conformationally
dynamic 1 , 2 , 3 , 4 , 5 , 6 , 7 – 8 . Imaging by single-molecule fluorescence resonance energy …

We describe a protocol for the rapid labeling of cell-surface proteins in living mammalian
cells using click chemistry. The labeling method is based on strain-promoted alkyne-azide …