DeepCell Kiosk: scaling deep learning–enabled cellular image analysis with Kubernetes

…, N Greenwald, V Vijayakumar, B Chang, E Pao… - Nature …, 2021 - nature.com
Deep learning is transforming the analysis of biological images, but applying these models
to large datasets remains challenging. Here we describe the DeepCell Kiosk, cloud-native …

[HTML][HTML] Label-free isolation of prostate circulating tumor cells using Vortex microfluidic technology

C Renier, E Pao, J Che, HE Liu, CA Lemaire… - NPJ precision …, 2017 - nature.com
There has been increased interest in utilizing non-invasive “liquid biopsies” to identify
biomarkers for cancer prognosis and monitoring, and to isolate genetic material that can predict …

Accurate cell tracking and lineage construction in live-cell imaging experiments with deep learning

…, D Kyme, C Pavelchek, T Price, T Kudo, E Pao… - Biorxiv, 2019 - biorxiv.org
Live-cell imaging experiments have opened an exciting window into the behavior of living
systems. While these experiments can produce rich data, the computational analysis of these …

[HTML][HTML] Label-free enumeration, collection and downstream cytological and cytogenetic analysis of circulating tumor cells

M Dhar, E Pao, C Renier, DE Go, J Che, R Montoya… - Scientific reports, 2016 - nature.com
Circulating tumor cells (CTCs) have a great potential as indicators of metastatic disease that
may help physicians improve cancer prognostication, treatment and patient outcomes. …

Scaling biological discovery at the interface of deep learning and cellular imaging

…, E Pradhan, A Ates, D Gallandt, R Barnowski, E Pao… - Nature …, 2023 - nature.com
Concurrent advances in imaging technologies and deep learning have transformed the nature
and scale of data that can now be collected with imaging. Here we discuss the progress …

Quantitative magnetic separation of particles and cells using gradient magnetic ratcheting

C Murray, E Pao, P Tseng, S Aftab, R Kulkarni, M Rettig… - Small, 2016 - Wiley Online Library
Extraction of rare target cells from biosamples is enabling for life science research. Traditional
rare cell separation techniques, such as magnetic activated cell sorting, are robust but …

Unsupervised capture and profiling of rare immune cells using multi-directional magnetic ratcheting

C Murray, H Miwa, M Dhar, DE Park, E Pao, J Martinez… - Lab on a Chip, 2018 - pubs.rsc.org
Immunotherapies (IT) require induction, expansion, and maintenance of specific changes to
a patient's immune cell repertoire which yield a therapeutic benefit. Recently, mechanistic …

Continuous and quantitative purification of T-cell subsets for cell therapy manufacturing using magnetic ratcheting cytometry

C Murray, E Pao, A Jann, DE Park… - SLAS TECHNOLOGY …, 2018 - journals.sagepub.com
Edward Pao, and Dino Di Carlo hold equity in Ferrologix, Inc., which has licensed technology
described in this article. … Coleman Murray and Edward Pao contributed equally to this work. …

Dynamic allocation of computational resources for deep learning-enabled cellular image analysis with Kubernetes

…, N Koe, D Kyme, T Kudo, B Chang, E Pao… - BioRxiv, 2018 - biorxiv.org
Deep learning is transforming the ability of life scientists to extract information from images.
These techniques have better accuracy than conventional approaches and enable …

[HTML][HTML] A Foundation Model for Cell Segmentation

…, M Marks, R Dilip, Q Li, M Schwartz, E Pradhan, E Pao… - bioRxiv, 2023 - ncbi.nlm.nih.gov
Cells are the fundamental unit of biological organization, and identifying them in imaging
data-cell segmentation-is a critical task for various cellular imaging experiments. While deep …