Photoredox‐Catalyzed Labeling of Hydroxyindoles with Chemoselectivity (PhotoCLIC) for Site‐Specific Protein Bioconjugation

…, D Jewel, SE Canarelli, ED Ficaretta… - Angewandte Chemie …, 2023 - Wiley Online Library
We have developed a novel visible‐light‐catalyzed bioconjugation reaction, PhotoCLIC,
that enables chemoselective attachment of diverse aromatic amine reagents onto a site‐…

A Facile Platform to Engineer Escherichia coli Tyrosyl-tRNA Synthetase Adds New Chemistries to the Eukaryotic Genetic Code, Including a Phosphotyrosine Mimic

…, C Soni, CM Hillenbrand, ED Ficaretta… - ACS central …, 2022 - ACS Publications
The Escherichia coli tyrosyl-tRNA synthetase (EcTyrRS)/tRNA EcTyr pair offers an attractive
platform for genetically encoding new noncanonical amino acids (ncAA) in eukaryotes. …

Structural robustness affects the engineerability of aminoacyl-tRNA synthetases for genetic code expansion

KT Grasso, MJR Yeo, CM Hillenbrand, ED Ficaretta… - Biochemistry, 2021 - ACS Publications
The ability to engineer the substrate specificity of natural aminoacyl-tRNA synthetase/tRNA
pairs facilitates the site-specific incorporation of noncanonical amino acids (ncAAs) into …

Electrochemical labelling of hydroxyindoles with chemoselectivity for site-specific protein bioconjugation

…, SE Canarelli, A Mondal, D Jewel, ED Ficaretta… - Nature Chemistry, 2023 - nature.com
Electrochemistry has recently emerged as a powerful approach in small-molecule synthesis
owing to its numerous attractive features, including precise control over the fundamental …

A robust platform for unnatural amino acid mutagenesis in E. coli using the bacterial tryptophanyl-tRNA synthetase/tRNA pair

ED Ficaretta, CJJ Wrobel, SJS Roy, SB Erickson… - Journal of molecular …, 2022 - Elsevier
We report the development of a robust user-friendly Escherichia coli (E. coli) expression
system, derived from the BL21(DE3) strain, for site-specifically incorporating unnatural amino …

[HTML][HTML] Expanding the substrate scope of pyrrolysyl-transfer RNA synthetase enzymes to include non-α-amino acids in vitro and in vivo

…, LT Roe, NX Hamlish, B Shah, Z Zhang, E Ficaretta… - Nature …, 2023 - nature.com
… An asterisk indicates the peak that corresponds to the indicated acyl-tRNA while a D
indicates the peak that corresponds to the decarboxylation of the malonyl-tRNA. d, Heat map …

Substitution of the sole tryptophan of the cupredoxin, amicyanin, with 5-hydroxytryptophan alters fluorescence properties and energy transfer to the type 1 copper site

AJ Pastore, E Ficaretta, A Chatterjee… - Journal of inorganic …, 2022 - Elsevier
Amicyanin is a type 1 copper protein with a single tryptophan residue. Using genetic code
expansion, the tryptophan was selectively replaced with the unnatural amino acid, 5-…

Resolving the Limitations of Genetic Code Expansion Platforms

KT Grasso - 2021 - search.proquest.com
Over the past twenty years, the site-specific incorporation of unnatural amino acids (UAAs)
into a target protein through genetic code expansion (GCE) has emerged as one of the …

[BOOK][B] Culture creole: imprenditrici straniere a Milano

C Lunghi - 2003 - books.google.com
… al contenuto simbolico incorporato: si tratta del sistema massmediatico, cioè l'industria
culturale classica, del sistema della produzione artistica o artigianale per un pubblico d'élite, e …