Bacillus thuringiensis (Bt) is a natural crystal-making bacterium. Bt diversified into many
subspecies that have evolved to produce crystals of hundreds of pesticidal proteins with …

“Smart fluorophores”, such as reversibly switchable fluorescent proteins, are crucial for
advanced fluorescence imaging. However, only a limited number of such labels is available, and …

Photolyase is an enzyme that uses light to catalyze DNA repair. To capture the reaction
intermediates involved in the enzyme’s catalytic cycle, we conducted a time-resolved …

Advanced imaging techniques crucially depend on the labels used. In this work, we present
the structure-guided design of a fluorescent protein that displays both reversibly …

Unstable states studied in kinetic, time-resolved and ligand-based crystallography are often
characterized by a low occupancy, which hinders structure determination by conventional …

Successful co‐crystallization of a noncovalent complex between hen egg‐white lysozyme (HEWL)
and the monomeric Zr IV ‐substituted Keggin polyoxometalate (POM) (Zr1 K1), (Et 2 …

The effect of the protein environment on the formation and stabilization of an elusive catalytically
active polyoxometalate (POM) species, K 6 [Hf(α 2 ‐P 2 W 17 O 61 )] (1), is reported. In …

Green-to-red photoconvertible fluorescent proteins repeatedly enter dark states, causing
interrupted tracks in single-particle-tracking localization microscopy (sptPALM). We identified a …

Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus
thuringiensis subsp. israelensis and jegathesan, respectively. The toxins naturally crystallize …

Green-to-red photoconvertible fluorescent proteins (PCFPs) are key players in advanced
microscopy schemes such as photoactivated localization microscopy (PALM). Whereas …