… We thank members of the De La Cruz and Ostap laboratories for helpful discussions, and
Lee Ligon and Chris Yengo for comments on the manuscript. EDLC is supported by grants …

… 1c; De La Cruz 2005). Efficient filament severing is readily observed at <1 μM cofilin (…
the filament lattice and promote fragmentation (De La Cruz 2005). Mathematical models that …

RNA helicase enzymes catalyze the in vivo folding and conformational re-arrangement of
RNA. DEAD-box proteins (DBPs) make up the largest family of RNA helicases and are found …

… Weak-binding myosin states dynamically detach and rebind to actin with a low affinity (K d
> 1 μM), whereas the strong-binding myosin states remain bound to … Enrique M. De La Cruz …

The organization of actin filaments into higher-ordered structures governs eukaryotic cell
shape and movement. Global actin network size and architecture are maintained in a dynamic …

Three methods, fluorescence anisotropy of rhodamine-labeled profilin, intrinsic fluorescence
and nucleotide exchange, give the same affinity, K d = 0.1 μM, for Acanthamoeba profilins …

Myosin V is a double-headed processive molecular motor that moves along an actin
filament by taking 36-nm steps. Using optical trapping nanometry with high spatiotemporal …

Diseases of ectopic calcification of the vascular wall range from lethal orphan diseases
such as generalized arterial calcification of infancy (GACI), to common diseases such as …

The assembly of actin monomers into filaments and networks plays vital roles throughout
eukaryotic biology, including intracellular transport, cell motility, cell division, determining …

We investigate, using molecular dynamics, how the severing protein, actin depolymerization
factor (ADF)/cofilin, modulates the structure, conformational dynamics, and mechanical …