Computational design of transmembrane pores
Transmembrane channels and pores have key roles in fundamental biological processes 1
and in biotechnological applications such as DNA nanopore sequencing 2 , 3 – 4 , resulting …
and in biotechnological applications such as DNA nanopore sequencing 2 , 3 – 4 , resulting …
Human CTP synthase filament structure reveals the active enzyme conformation
EM Lynch, DR Hicks, M Shepherd, JA Endrizzi… - Nature structural & …, 2017 - nature.com
The universally conserved enzyme CTP synthase (CTPS) forms filaments in bacteria and
eukaryotes. In bacteria, polymerization inhibits CTPS activity and is required for nucleotide …
eukaryotes. In bacteria, polymerization inhibits CTPS activity and is required for nucleotide …
[PDF][PDF] Selective activation of PFKL suppresses the phagocytic oxidative burst
…, MP Cooper, MA Voronkova, BA Webb, EM Lynch… - Cell, 2021 - cell.com
In neutrophils, nicotinamide adenine dinucleotide phosphate (NADPH) generated via the
pentose phosphate pathway fuels NADPH oxidase NOX2 to produce reactive oxygen species …
pentose phosphate pathway fuels NADPH oxidase NOX2 to produce reactive oxygen species …
Coupled structural transitions enable highly cooperative regulation of human CTPS2 filaments
EM Lynch, JM Kollman - Nature structural & molecular biology, 2020 - nature.com
Many enzymes assemble into defined oligomers, providing a mechanism for cooperatively
regulating activity. Recent studies have described a mode of regulation in which enzyme …
regulating activity. Recent studies have described a mode of regulation in which enzyme …
Cryo-EM structures of CTP synthase filaments reveal mechanism of pH-sensitive assembly during budding yeast starvation
JM Hansen, A Horowitz, EM Lynch, DP Farrell… - Elife, 2021 - elifesciences.org
Many metabolic enzymes self-assemble into micron-scale filaments to organize and regulate
metabolism. The appearance of these assemblies often coincides with large metabolic …
metabolism. The appearance of these assemblies often coincides with large metabolic …
Filament formation by metabolic enzymes—A new twist on regulation
EM Lynch, JM Kollman, BA Webb - Current opinion in cell biology, 2020 - Elsevier
Compartmentalization of metabolic enzymes through protein–protein interactions is an
emerging mechanism for localizing and regulating metabolic activity. Self-assembly into linear …
emerging mechanism for localizing and regulating metabolic activity. Self-assembly into linear …
[PDF][PDF] Activation of the γ-tubulin complex by the Mto1/2 complex
EM Lynch, LM Groocock, WE Borek, KE Sawin - Current Biology, 2014 - cell.com
The multisubunit γ-tubulin complex (γ-TuC) is critical for microtubule nucleation in eukaryotic
cells [1, 2], but it remains unclear how the γ-TuC becomes active specifically at microtubule-…
cells [1, 2], but it remains unclear how the γ-TuC becomes active specifically at microtubule-…
Polymerization in the actin ATPase clan regulates hexokinase activity in yeast
PR Stoddard, EM Lynch, DP Farrell, AM Dosey… - Science, 2020 - science.org
The actin fold is found in cytoskeletal polymers, chaperones, and various metabolic enzymes.
Many actin-fold proteins, such as the carbohydrate kinases, do not polymerize. We found …
Many actin-fold proteins, such as the carbohydrate kinases, do not polymerize. We found …
Structural basis for isoform-specific inhibition of human CTPS1
EM Lynch, MA DiMattia, S Albanese… - Proceedings of the …, 2021 - National Acad Sciences
Cytidine triphosphate synthase 1 (CTPS1) is necessary for an effective immune response,
as revealed by severe immunodeficiency in CTPS1-deficient individuals [E. Martin et al.], […
as revealed by severe immunodeficiency in CTPS1-deficient individuals [E. Martin et al.], […
[HTML][HTML] De novo design of pH-responsive self-assembling helical protein filaments
Biological evolution has led to precise and dynamic nanostructures that reconfigure in
response to pH and other environmental conditions. However, designing micrometre-scale …
response to pH and other environmental conditions. However, designing micrometre-scale …