The mammalian cilium protrudes from the apical/lumenal surface of polarized cells and acts
as a sensor of environmental cues. Numerous developmental disorders and pathological …

Since their introduction, the interaction trap and other two-hybrid systems have been used to
study protein-protein interactions. Despite their general use, little is known about the extent …

Temporally and spatially controlled activation of the Aurora A kinase (AURKA) regulates
centrosome maturation, entry into mitosis, formation and function of the bipolar spindle, and …

Background Cisplatin-based neoadjuvant chemotherapy (NAC) before cystectomy is the
standard of care for muscle-invasive bladder cancer (MIBC), with 25–50% of patients expected …

This unit presents protocols designed to detect interacting proteins. Using yeast as a “test
tube” and transcriptional activation of a reporter system, interacting proteins can be identified. …

The tuberous sclerosis complex 2 (TSC2) is a tumor suppressor gene that plays a causative
role in the autosomal dominant syndrome of tuberous sclerosis. The latter is characterized …

The DNA mismatch repair (MMR) is a specialized system, highly conserved throughout
evolution, involved in the maintenance of genomic integrity. To identify novel human genes that …

Cilia are microtubule-based structures that protrude from the cell surface and function as
sensors for mechanical and chemical environmental cues that regulate cellular differentiation or …

Aberrant gene expression that drives human cancer can arise from epigenetic dysregulation.
Although much attention has focused on altered activity of transcription factors and …

Although HEF1 has a well-defined role in integrin-dependent attachment signalling at focal
adhesions, it relocalizes to the spindle asters at mitosis. We report here that overexpression …