Fluorescent tools have revolutionized our ability to probe biological dynamics, particularly at
the cellular level. Fluorescent sensors have been developed on several platforms, utilizing …

RNAs directly regulate a vast array of cellular processes, emphasizing the need for robust
approaches to fluorescently label and track RNAs in living cells. Here, we develop an RNA …

Protein folding is an essential prerequisite for protein function and hence cell function.
Kinetic and thermodynamic studies of small proteins that refold reversibly were essential for …

The central dogma teaches us that DNA makes RNA, which in turn makes proteins, the
main building blocks of the cell. But this over simplified linear transmission of information …

To evaluate the physical parameters governing translocation of an unfolded protein across
a lipid bilayer, we studied protein transport through aerolysin, a passive protein channel, at …

Visualization of RNAs in live cells is critical to understand biology of RNA dynamics and
function in the complex cellular environment. Detection of RNAs with a fluorescent marker …

Various fluorescent probes have been developed to reveal the biological functions of intracellular
labile Zn 2+ . Here, we present Green Zinc Probe (GZnP), a novel genetically encoded …

Autotransporter ( AT ) proteins provide a diverse array of important virulence functions to G
ram‐negative bacterial pathogens, and have also been adapted for protein surface display …

Autotransporter (AT) proteins are the largest class of extracellular virulence proteins secreted
from Gram-negative bacteria. The mechanism by which AT proteins cross the bacterial …

Autotransporter (AT) proteins are a broad class of virulence proteins from Gram-negative
bacterial pathogens that require their own C-terminal transmembrane domain to translocate …