This paper presents a generalisable method for the calibration of fluorescence readings on
microplate readers, in order to convert arbitrary fluorescence units into absolute units. …

Life on Earth is incredibly diverse. Yet, underneath that diversity, there are a number of constants
and highly conserved processes: all life is based on DNA and RNA; the genetic code is …

Artificial metalloenzymes (ArMs hereafter) combine attractive features of both homogeneous
catalysts and enzymes and offer the potential to implement new-to-nature reactions in living …

Biocontainment comprises any strategy applied to ensure that harmful organisms are
confined to controlled laboratory conditions and not allowed to escape into the environment. …

As demand for the automation of biological assays has increased over recent years, the range
of measurement types implemented by multiwell plate readers has broadened and the list …

Translational readthrough—suppression of termination at a stop codon—is exploited in the
replication cycles of several viruses and represents a potential target for antiviral intervention. …

Directed evolution has been remarkably successful at expanding the chemical and functional
boundaries of biology. That progress is heavily dependent on the robustness and flexibility …

This paper presents a generalisable method for the calibration of fluorescence readings on
microplate readers, in order to convert arbitrary fluorescence units into absolute units. …

A promising route to tackle the trade-off in cellular resources between synthetic protein
production and cellular growth is to use a separate dedicated pool of orthogonal ribosomes to …

While inter-lab calibration standards are approaching mainstream usage in synthetic biology,
such calibrations are not in fact sufficient for absolute protein quantification required for …