Over the past 3 years, bacterial transcriptomics has undergone a massive revolution.
Increased sequencing capacity and novel tools have made it possible to explore the bacterial …

Cells respond to their environment by sensing signals and translating them into changes in
gene expression. In recent years, synthetic networks have been designed in both prokaryotic …

The genome of Mycoplasma pneumoniae is among the smallest found in self-replicating
organisms. To study the basic principles of bacterial proteome organization, we used tandem …

To study basic principles of transcriptome organization in bacteria, we analyzed one of the
smallest self-replicating organisms, Mycoplasma pneumoniae. We combined strand-specific …

To understand basic principles of bacterial metabolism organization and regulation, but
also the impact of genome size, we systematically studied one of the smallest bacteria, …

Protein post‐translational modifications (PTMs) represent important regulatory states that
when combined have been hypothesized to act as molecular codes and to generate a …

Identifying all essential genomic components is critical for the assembly of minimal artificial
life. In the genome‐reduced bacterium Mycoplasma pneumoniae, we found that small ORF s (…

DNA-binding proteins are central regulators of chromosome organization; however, in
genome-reduced bacteria their diversity is largely diminished. Whether the chromosomes of such …

Mycoplasma pneumoniae, a threatening pathogen with a minimal genome, is a model organism
for bacterial systems biology for which substantial experimental information is available. …

Translation efficiency has been mainly studied by ribosome profiling, which only provides an
incomplete picture of translation kinetics. Here, we integrated the absolute quantifications of …