Hypothesis–buttressed rings assemble, clamp, and release SNAREpins for synaptic transmission

…, SS Krishnakumar, K Grushin, F Pincet - FEBS …, 2017 - Wiley Online Library
… we will take the lower bound for the unknown force exerted by each SNAREpin to be F trans
… force, f, that contributes to the movement is the projection of the total force, F, tangentially to …

[HTML][HTML] Recent applications of fluorescence recovery after photobleaching (FRAP) to membrane bio-macromolecules

G Rayan, JE Guet, N Taulier, F Pincet, W Urbach - Sensors, 2010 - mdpi.com
This review examines some recent applications of fluorescence recovery after photobleaching
(FRAP) to biopolymers, while mainly focusing on membrane protein studies. Initially, we …

Turbocharging synaptic transmission

JE Rothman, K Grushin, M Bera, F Pincet - FEBS letters, 2023 - Wiley Online Library
Evidence from biochemistry, genetics, and electron microscopy strongly supports the idea
that a ring of Synaptotagmin is central to the clamping and release of synaptic vesicles (SVs) …

Force measurements in E-cadherin–mediated cell doublets reveal rapid adhesion strengthened by actin cytoskeleton remodeling through Rac and Cdc42

YS Chu, WA Thomas, O Eder, F Pincet… - The Journal of cell …, 2004 - rupress.org
… 6 F), we suspect that Rac may affect cadherin activity directly, independently of actin
remodeling, by an unknown mechanism. We do not exclude the possibility that activated Rac also …

Energetics and dynamics of SNAREpin folding across lipid bilayers

F Li, F Pincet, E Perez, WS Eng, TJ Melia… - Nature structural & …, 2007 - nature.com
… On the profile shown in Figure 1, this adhesive jump occurred at around 8 nm with a pull-off
force, F t/v /R (where F is force, t/v denotes the use of a t-SNARE/v-SNARE pair and R is …

Influence of pH on stability and dynamic properties of asphaltenes and other amphiphilic molecules at the oil− water interface

S Poteau, JF Argillier, D Langevin, F Pincet… - Energy & …, 2005 - ACS Publications
Oil-in-water emulsions are currently being investigated to facilitate the transport of viscous
heavy oils. The behavior of these emulsions is largely controlled by the interfaces between oil …

Arf1/COPI machinery acts directly on lipid droplets and enables their connection to the ER for protein targeting

…, J Wang, R Beck, TJ Gould, ES Allgeyer, F Pincet… - elife, 2014 - elifesciences.org
… From the inner diameter d of the injection tube (d = 250 µm), the surface tension is determined
by mg/(π*d*f) where f is a Wilkinson geometric parameter correction that depends on the …

SNARE proteins: one to fuse and three to keep the nascent fusion pore open

…, J Wang, HW Wang, TJ Melia, JE Rothman, F Pincet - Science, 2012 - science.org
Neurotransmitters are released through nascent fusion pores, which ordinarily dilate after
bilayer fusion, preventing consistent biochemical studies. We used lipid bilayer nanodiscs as …

Johnson-Kendall-Roberts theory applied to living cells

YS Chu, S Dufour, JP Thiery, E Perez, F Pincet - Physical review letters, 2005 - APS
Johnson-Kendall-Roberts (JKR) theory is an accurate model for strong adhesion energies of
soft slightly deformable material. Little is known about the validity of this theory on complex …

Control of plasma membrane lipid homeostasis by the extended synaptotagmins

…, Y Sawaki, MA Surma, C Klose, F Pincet… - Nature cell …, 2016 - nature.com
… Right, c: values of ΔF/F 0 corresponding to the end of the experiment … (f) Values of F/F 0
corresponding to the end of the … (j) Values of ΔF/F 0 corresponding to the end of the incubation as …