Class 2 CRISPR-Cas nucleases are programmable genome editing tools with promising
applications in human health and disease. However, DNA cleavage at off-target sites that …

CRISPR-Cas nucleoproteins target foreign DNA via base pairing with a crRNA. However, a
quantitative description of protein binding and nuclease activation at off-target DNA …

Genome engineering nucleases must access chromatinized DNA. Here, we investigate how
AsCas12a cleaves DNA within human nucleosomes and phase-condensed nucleosome …

10.7554/eLife.20340.001 The replicative lifespan (RLS) of a cell—defined as the number of
cell divisions before death—has informed our understanding of the mechanisms of cellular …

Animals with complex nervous systems demand sleep for memory consolidation and synaptic
remodeling. Here, we show that, although the Caenorhabditis elegans nervous system …

In the emerging field of whole-brain imaging at single-cell resolution, which represents one
of the new frontiers to investigate the link between brain activity and behavior, the nematode …

Fluorescent labeling of proteins is a critical requirement for single-molecule imaging studies.
Many protein labeling strategies require harsh conditions or large epitopes that can …

Memory, defined as an alteration in behavior towards a stimulus that follows as a consequence
of experience, arises when a sensory stimulus is encountered at the same time that the …

Memory is one of the most important abilities of the brain. It is defined as an alteration in
behavior as a consequence of an experience. For example, the C. elegans nematode will …

Animals with complex nervous systems demand sleep for memory consolidation and synaptic
remodeling. Here we show that though the Caenorhabditis elegans nervous system has a …