The type II clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated
(Cas) system has emerged recently as a powerful method to manipulate the …

Genetic screens are powerful tools for the functional annotation of genomes. In the context of
multicellular organisms, interrogation of gene function is greatly facilitated by methods that …

We present tRNA-based vectors for producing multiple clustered regularly interspaced short
palindromic repeats (CRISPR) single guide RNAs (sgRNAs) from a single RNA polymerase …

Proteins of the Wnt family are secreted signaling molecules that regulate multiple processes
in animal development and control tissue homeostasis in the adult. Wnts spread over …

Wnt ligands are lipid-modified, secreted glycoproteins that control multiple steps during
embryogenesis and adult-tissue homeostasis. Little is known about the mechanisms underlying …

In the last decade, RNA interference (RNAi), a cellular mechanism that uses RNA-guided
degradation of messenger RNA transcripts, has had an important impact on identifying and …

CRISPR-Cas genome engineering has revolutionized biomedical research by enabling
targeted genome modification with unprecedented ease. In the popular model organism …

Wnt proteins are lipid-modified glycoproteins that play a central role in development, adult
tissue homeostasis and disease. Secretion of Wnt proteins is mediated by the Wnt-binding …

Gene drive systems promote the spread of genetic elements through populations by assuring
they are inherited more often than Mendelian segregation would predict (see the figure). …

The Piwi-interacting RNA (piRNA) pathway plays an essential role in the repression of
transposons in the germline. Other functions of piRNAs such as post-transcriptional regulation of …