Monocytes and macrophages have crucial and distinct roles in tissue homeostasis and
immunity, but they also contribute to a broad spectrum of pathologies and are thus attractive …

Despite accumulating evidence suggesting local self-maintenance of tissue macrophages
in the steady state, the dogma remains that tissue macrophages derive from monocytes. …

Microglia are the resident macrophages of the central nervous system and are associated
with the pathogenesis of many neurodegenerative and brain inflammatory diseases; however, …

Defining the origins and developmental pathways of tissue-resident macrophages should
help refine our understanding of the role of these cells in various disease settings and enable …

Microglia are the resident macrophage population of the central nervous system (CNS).
Adequate microglial function is crucial for a healthy CNS. Microglia are not only the first immune …

Macrophages are the first cells of the nascent immune system to emerge during embryonic
development. In mice, embryonic macrophages infiltrate developing organs, where they …

Advances in single-cell technologies have enabled high-resolution dissection of tissue
composition. Several tools for dimensionality reduction are available to analyze the large number …

Breast cancers are complex cellular ecosystems where heterotypic interactions play central
roles in disease progression and response to therapy. However, our knowledge of their …

Microglia, the resident immune cells of the brain, rapidly change states in response to their
environment, but we lack molecular and functional signatures of different microglial …

These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical aspects of …