User profiles for Francisco A. Leone

Francisco Assis Leone

Professor de Bioquímica, Universidade de São Paulo
Verified email at ffclrp.usp.br
Cited by 3524

SigrafW: An easy‐to‐use program for fitting enzyme kinetic data

FA Leone, JA Baranauskas… - Biochemistry and …, 2005 - Wiley Online Library
SigrafW is Windows‐compatible software developed using the Microsoft® Visual Basic
Studio program that uses the simplified Hill equation for fitting kinetic data from allosteric and …

[HTML][HTML] Purification and biochemical characterization of a mycelial glucose-and xylose-stimulated β-glucosidase from the thermophilic fungus Humicola insolens

…, CV Nascimento, JC Rosa, DC Masui, FA Leone… - Process …, 2010 - Elsevier
A mycelial β-glucosidase from the thermophilic mold Humicola insolens was purified and
biochemically characterized. The enzyme showed carbohydrate content of 21% and apparent …

Effects of ammonia stress in the Amazon river shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae)

…, RO Faleiros, EA Almeida, JC McNamara, FA Leone - Aquatic Toxicology, 2016 - Elsevier
We evaluate the effects of total ammonia nitrogen-N (TAN) exposure for 72 h on (Na + ,K + )-
and V(H + )-ATPase activities and on their subunit expressions in gills of the diadromous …

Purification and biochemical properties of a glucose-stimulated β-D-glucosidase produced by Humicola grisea var. thermoidea grown on sugarcane bagasse

…, FHM Souza, DC Masui, FA Leone… - The journal of …, 2010 - Springer
The effect of several carbon sources on the production of mycelial-bound β-glucosidase by
Humicola grisea var. thermoidea in submerged fermentation was investigated. Maximum …

Hemolymph ionic regulation and adjustments in gill (Na+, K+)-ATPase activity during salinity acclimation in the swimming crab Callinectes ornatus (Decapoda …

…, RPM Furriel, JC McNamara, FA Leone - … and Physiology Part A …, 2009 - Elsevier
We evaluate hemolymph osmotic and ionic regulatory abilities and characterize a posterior
gill microsomal (Na+, K+)-ATPase from the marine swimming crab, Callinectes ornatus, …

Alkaline phosphatase from rat osseous plates: purification and biochemical characterization of a soluble form

…, K Ciuffi, RPM Furriel, P Ciancaglini, FA Leone - … et Biophysica Acta (BBA …, 1991 - Elsevier
A soluble form of an alkaline phosphatase obtained from rat osseous plates was purified
204-fold with a yield of 24.3%. The purified enzyme showed a single protein band of M r 80 000 …

Gill ion transport ATPases and ammonia excretion in aquatic crustaceans

FA Leone, MN Lucena, DP Garçon, MR Pinto… - Acid-Base Balance and …, 2017 - Springer
Crustaceans inhabit diverse biotopes, often subject to alterations that constitute a severe
challenge to their homeostatic mechanisms. These challenges have driven the evolution of …

Effect of molecular surface packing on the enzymatic activity modulation of an anchored protein on phospholipid Langmuir monolayers

…, RG Oliveira, DC Masui, RPM Furriel, FA Leone… - Langmuir, 2005 - ACS Publications
The catalytic activity of a glycosylphosphatidylinositol (GPI)-anchored alkaline phosphatase
has been studied in Langmuir phospholipid monolayers at different surface pressures. The …

A biotinylated conducting polypyrrole for the spatially controlled construction of an amperometric biosensor

…, A Senillou, H Perrot, RPM Furriel, FA Leone - Analytical …, 1999 - ACS Publications
A new biotin derivative functionalized by an electropolymerizable pyrrole group has been
synthesized. The electrooxidation of this biotin pyrrole has allowed the formation of …

Gill-specific (Na+, K+)-ATPase activity and α-subunit mRNA expression during low-salinity acclimation of the ornate blue crab Callinectes ornatus (Decapoda …

FA Leone, DP Garçon, MN Lucena, RO Faleiros… - … and Physiology Part B …, 2015 - Elsevier
We evaluate (Na + , K + )-ATPase activity, and protein and gene expression of the α-subunit
in posterior gills 6 and 7 of Callinectes ornatus, a euryhaline crab, during a 10-day …