User profiles for Francisco A. Leone
Francisco Assis LeoneProfessor de Bioquímica, Universidade de São Paulo Verified email at ffclrp.usp.br Cited by 3524 |
SigrafW: An easy‐to‐use program for fitting enzyme kinetic data
FA Leone, JA Baranauskas… - Biochemistry and …, 2005 - Wiley Online Library
SigrafW is Windows‐compatible software developed using the Microsoft® Visual Basic
Studio program that uses the simplified Hill equation for fitting kinetic data from allosteric and …
Studio program that uses the simplified Hill equation for fitting kinetic data from allosteric and …
[HTML][HTML] Purification and biochemical characterization of a mycelial glucose-and xylose-stimulated β-glucosidase from the thermophilic fungus Humicola insolens
A mycelial β-glucosidase from the thermophilic mold Humicola insolens was purified and
biochemically characterized. The enzyme showed carbohydrate content of 21% and apparent …
biochemically characterized. The enzyme showed carbohydrate content of 21% and apparent …
Effects of ammonia stress in the Amazon river shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae)
We evaluate the effects of total ammonia nitrogen-N (TAN) exposure for 72 h on (Na + ,K + )-
and V(H + )-ATPase activities and on their subunit expressions in gills of the diadromous …
and V(H + )-ATPase activities and on their subunit expressions in gills of the diadromous …
Purification and biochemical properties of a glucose-stimulated β-D-glucosidase produced by Humicola grisea var. thermoidea grown on sugarcane bagasse
The effect of several carbon sources on the production of mycelial-bound β-glucosidase by
Humicola grisea var. thermoidea in submerged fermentation was investigated. Maximum …
Humicola grisea var. thermoidea in submerged fermentation was investigated. Maximum …
Hemolymph ionic regulation and adjustments in gill (Na+, K+)-ATPase activity during salinity acclimation in the swimming crab Callinectes ornatus (Decapoda …
We evaluate hemolymph osmotic and ionic regulatory abilities and characterize a posterior
gill microsomal (Na+, K+)-ATPase from the marine swimming crab, Callinectes ornatus, …
gill microsomal (Na+, K+)-ATPase from the marine swimming crab, Callinectes ornatus, …
Alkaline phosphatase from rat osseous plates: purification and biochemical characterization of a soluble form
A soluble form of an alkaline phosphatase obtained from rat osseous plates was purified
204-fold with a yield of 24.3%. The purified enzyme showed a single protein band of M r 80 000 …
204-fold with a yield of 24.3%. The purified enzyme showed a single protein band of M r 80 000 …
Gill ion transport ATPases and ammonia excretion in aquatic crustaceans
Crustaceans inhabit diverse biotopes, often subject to alterations that constitute a severe
challenge to their homeostatic mechanisms. These challenges have driven the evolution of …
challenge to their homeostatic mechanisms. These challenges have driven the evolution of …
Effect of molecular surface packing on the enzymatic activity modulation of an anchored protein on phospholipid Langmuir monolayers
…, RG Oliveira, DC Masui, RPM Furriel, FA Leone… - Langmuir, 2005 - ACS Publications
The catalytic activity of a glycosylphosphatidylinositol (GPI)-anchored alkaline phosphatase
has been studied in Langmuir phospholipid monolayers at different surface pressures. The …
has been studied in Langmuir phospholipid monolayers at different surface pressures. The …
A biotinylated conducting polypyrrole for the spatially controlled construction of an amperometric biosensor
A new biotin derivative functionalized by an electropolymerizable pyrrole group has been
synthesized. The electrooxidation of this biotin pyrrole has allowed the formation of …
synthesized. The electrooxidation of this biotin pyrrole has allowed the formation of …
Gill-specific (Na+, K+)-ATPase activity and α-subunit mRNA expression during low-salinity acclimation of the ornate blue crab Callinectes ornatus (Decapoda …
We evaluate (Na + , K + )-ATPase activity, and protein and gene expression of the α-subunit
in posterior gills 6 and 7 of Callinectes ornatus, a euryhaline crab, during a 10-day …
in posterior gills 6 and 7 of Callinectes ornatus, a euryhaline crab, during a 10-day …