User profiles for Frederick S. Gimble
Frederick GimbleAssociate Professor, Department of Biochemistry, Purdue University Verified email at purdue.edu Cited by 3093 |
Protein splicing elements: inteins and exteins--a definition of terms and recommended nomenclature.
FB Perler, EO Davis, GE Dean, FS Gimble… - Nucleic acids …, 1994 - ncbi.nlm.nih.gov
INTRODUCTION Several archaeal, eubacterial and eucaryotic genes have been identified
with in-frame insertions that are excised at the protein level, not at the RNA level (1-13, 24, …
with in-frame insertions that are excised at the protein level, not at the RNA level (1-13, 24, …
Homing of a DNA endonuclease gene by meiotic gene conversion in Saccharomyces cerevisiae
An unusual protein splicing reaction joins the N-terminal segment (A) and the C-terminal
segment (C) of the 119K primary translation product (ABC) of the yeast VMA1 gene to yield a …
segment (C) of the 119K primary translation product (ABC) of the yeast VMA1 gene to yield a …
[HTML][HTML] Crystal structure of PI-SceI, a homing endonuclease with protein splicing activity
X Duan, FS Gimble, FA Quiocho - Cell, 1997 - cell.com
PI-SceI is a bifunctional yeast protein that propagates its mobile gene by catalyzing protein
splicing and site-specific DNA double-strand cleavage. Here, we report the 2.4 Ć crystal …
splicing and site-specific DNA double-strand cleavage. Here, we report the 2.4 Ć crystal …
Invasion of a multitude of genetic niches by mobile endonuclease genes
FS Gimble - FEMS Microbiology Letters, 2000 - academic.oup.com
Persistence of a mobile DNA element in a population reflects a balance between the ability
of the host to eliminate the element and the ability of the element to survive and to …
of the host to eliminate the element and the ability of the element to survive and to …
The crystal structure of the gene targeting homing endonuclease I-SceI reveals the origins of its target site specificity
CM Moure, FS Gimble, FA Quiocho - Journal of molecular biology, 2003 - Elsevier
The I-SceI homing endonuclease enhances gene targeting by introducing double-strand
breaks at specific chromosomal loci, thereby increasing the recombination frequency. Here, we …
breaks at specific chromosomal loci, thereby increasing the recombination frequency. Here, we …
Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence
CM Moure, FS Gimble, FA Quiocho - Nature structural biology, 2002 - nature.com
The first X-ray structures of an intein–DNA complex, that of the two-domain homing
endonuclease PI-SceI bound to its 36-base pair DNA substrate, have been determined in the …
endonuclease PI-SceI bound to its 36-base pair DNA substrate, have been determined in the …
Engineering variants of the I-SceI homing endonuclease with strand-specific and site-specific DNA-nicking activity
Y Niu, K Tenney, H Li, FS Gimble - Journal of molecular biology, 2008 - Elsevier
The number of strand-specific nicking endonucleases that are currently available for laboratory
procedures and applications in vivo is limited, and none is sufficiently specific to nick …
procedures and applications in vivo is limited, and none is sufficiently specific to nick …
NgAgo possesses guided DNA nicking activity
Prokaryotic Argonautes (pAgos) have been proposed as more flexible tools for gene-editing
as they do not require sequence motifs adjacent to their targets for function, unlike popular …
as they do not require sequence motifs adjacent to their targets for function, unlike popular …
Substrate recognition and induced DNA distortion by the PI-SceI endonuclease, an enzyme generated by protein splicing
FS Gimble, J Wang - Journal of molecular biology, 1996 - Elsevier
… ) as a minimal recognition sequence for cleavage (Gimble & Thorner, 1993), but we have …
in vitro as a longer 67bp substrate (Gimble & Stephens, 1995) that includes extensive flanking …
in vitro as a longer 67bp substrate (Gimble & Stephens, 1995) that includes extensive flanking …
Assessing the plasticity of DNA target site recognition of the PI-SceI homing endonuclease using a bacterial two-hybrid selection system
The PI-SceI protein from Saccharomyces cerevisiae is a member of the LAGLIDADG family
of homing endonucleases that have been used in genomic engineering. To assess the …
of homing endonucleases that have been used in genomic engineering. To assess the …