[HTML][HTML] Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9

…, F Dingli, D Loew, E Rubinstein, G Boncompain… - Nature …, 2021 - nature.com
… (generated by FJ Verweij and G. Boncompain and available upon request to G. Van Niel:
guillaume.van-niel@inserm.fr). The CD63-SBP-pHluorin fragment was synthesized and ligated …

Synchronization of secretory protein traffic in populations of cells

G Boncompain, S Divoux, N Gareil, H De Forges… - Nature …, 2012 - nature.com
To dissect secretory traffic, we developed the retention using selective hooks (RUSH) system.
RUSH is a two-state assay based on the reversible interaction of a hook protein fused to …

The role of microtubules in secretory protein transport

…, AJ Jimenez, F Perez, G Boncompain - Journal of cell …, 2020 - journals.biologists.com
Microtubules are part of the dynamic cytoskeleton network and composed of tubulin dimers.
They are the main tracks used in cells to organize organelle positioning and trafficking of …

Local palmitoylation cycles define activity-regulated postsynaptic subdomains

Y Fukata, A Dimitrov, G Boncompain… - Journal of Cell …, 2013 - rupress.org
… 3 G), thus visualizing in real time depalmitoylation of synaptic PSD-95. This decrease
was not caused by photobleaching of GFP, as activity blockade by TTX under the same …

Transport and sorting in the Golgi complex: multiple mechanisms sort diverse cargo

G Boncompain, AV Weigel - Current opinion in cell biology, 2018 - Elsevier
G. Boncompain is working in the lab of F. Perez. Work in F. … Boncompain and AV Weigel
was funded by the Labex CellTisPhyBio and by the visitor program of HHMI — Janelia Research …

Photodynamic therapy with redaporfin targets the endoplasmic reticulum and Golgi apparatus

…, B Sveinbjørnsson, Ø Rekdal, G Boncompain… - The EMBO …, 2018 - embopress.org
Preclinical evidence depicts the capacity of redaporfin (Redp) to act as potent photosensitizer,
causing direct antineoplastic effects as well as indirect immune‐dependent destruction of …

The many routes of Golgi-dependent trafficking

G Boncompain, F Perez - Histochemistry and cell biology, 2013 - Springer
… They were found to be important for proteins bearing a C-terminal valine motif, like fusion
protein between VSV-G and CD8 or endogenous Frizzled-4, while having no effect on the …

RAB6 and microtubules restrict protein secretion to focal adhesions

…, D Pereira, F Perez, B Goud, G Boncompain… - Journal of Cell …, 2019 - rupress.org
… All the RUSH plasmids used in this study (except VSV-G-SBP-EGFP) use streptavidin-KDEL …
For permeabilization, cells were incubated in PBS supplemented with 2 g/liter BSA and 0.5 g

Production of Reactive Oxygen Species Is Turned On and Rapidly Shut Down in Epithelial Cells Infected with Chlamydia trachomatis

G Boncompain, B Schneider, C Delevoye… - Infection and …, 2010 - Am Soc Microbiol
… To synchronize the infection, cells with bacteria were centrifuged for 5 min at 385 × g (Allegra
6 KR; Beckman Coulter) and incubated for 1 h at 37C with 6% CO 2 . After 1 h of infection, …

Developmental regulation of apical endocytosis controls epithelial patterning in vertebrate tubular organs

…, J Bagwell, M Bosch-Fortea, G Boncompain… - Nature cell …, 2015 - nature.com
… (h) Quantification of Rab11 localization in g. Fluorescent intensity linear profiles were
drawn perpendicular to the centre of the apical plasma membrane (0 is the peak of apical F-actin …