… (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% (vol/vol) Nonidet-P40, 5 mM EDTA, 5 mM EGTA
and protease inhibitors), and the lysates were cleared by centrifugation (10 min at 16,000 g …

… Search parameters were set to a mass tolerance of 20 ppm for the precursor ions and 0.1
g/mol for the fragment ions. Two missed cleavage sites were allowed. Carbamidomethyl …

… ICP-MS analysis of the treated agar (7.3 цg/g Fe and 5.9 цg/g P) indicated a contribution
of 1.25 цM Fe and 1.875 цM P to the solid 1% agar medium. Images were analyzed using …

The analysis of ultralow input samples or even individual cells is essential to answering a
multitude of biomedical questions, but current proteomic workflows are limited in their …

… (H) Graph depicting the mean normalized difference in fluorescence intensity between the
bleached and unbleached regions from cells treated as in G. Error bars denote sem, n = 10 …

… ions within a given MS/MS spectrum, the G-score was calculated as the reciprocal value
of … /MS spectra with a G-score greater than 0.4 were derived from glycopeptides: After G-scoring, …

… This approach includes the g and A tensors as well as the electron-electron interactions
in the fitting process. The dipolar coupling D was calculated on the basis of the point-dipole …

… g for 3 min at 4C. The soluble supernatant was centrifuged for a further 20 min at 20000 g at
4… resuspension in 1 ml lysis buffer and centrifugation at 1000 g for 3 min at 4C. Washing was …

In the light of the ongoing single-cell revolution, scientific disciplines are combining forces to
retrieve as much relevant data as possible from trace amounts of biological material. For …

… g, h Tbl1x interactomes identified by mass spectrometry after 3 h PD (g) and 48 h CM/Fgf
withdrawal and CH treatment (h) in differentiating TSCs expressing Tbl1x-3xFlag and …