… The culture was pelleted at 5,000 g in glass Corex tubes, resuspended in distilled water,
and pelleted again at 5,000 g. After removing the supernatant, the pellets were dried for 24 h at …

Antibiotic development is frequently plagued by the rapid emergence of drug resistance.
However, assessing the risk of resistance development in the preclinical stage is difficult. …

Ribosomal RNA (rrn) operons, characteristically present in several copies in bacterial
genomes (7 in E. coli), play a central role in cellular physiology. We investigated the factors …

The ideal bacterial chassis provides a simplified, stable and predictable host environment
for synthetic biological circuits. Mutability and evolution can, however, compromise stability, …

Functional metagenomics is a powerful experimental tool to identify antibiotic resistance genes
(ARGs) in the environment, but the range of suitable host bacterial species is limited. This …

… -O-(benzotriazol-1-yl)uronium tetrafluoroborate) in order to prepare amides 5a-g. The nitro
groups of 5a-g were reduced by catalytic hydrogenation to give amines 6a-g. Compounds 6a-…

… To a stirred solution of compound 2 (3.08 g, 15.7 mmol) and triphenylphosphine (5.50 g,
21.0 mmol) in anhydrous tetrahydrofuran (200 mL) 1-Boc-4-hydroxypiperidine (4.0 g, 19.9 mmol…

… LB) medium was used (10 g tryptone, 5 g yeast extract, 5 g sodium chloride per liter of water)…
Terrific broth (TB; 24 g yeast extract, 12 g tryptone, 9.4 g K 2 HPO 4 , and 2 g KH 2 PO 4 per …

… 3 g beef extract, 17.5 g acid hydrolysate of casein, and 1.5 g starch… g of BHI powder (Carl
Roth GmbH) was dissolved in 1 L of water (7.5 g pig brain infusion, 10 g pig heart infusion, 10 g …

The emergence of multidrug‐resistant bacteria is a global health threat necessitating the
discovery of new antibacterials and novel strategies for fighting bacterial infections. We report …