… Work on mitosis in the Scholey laboratory is supported by NIH grant GM55507 and in the
Goshima laboratory by MEXT, HFSPO, the Inoue Foundation, the Uehara Foundation, and the …

… Ott for reagents; DB Jones and G. Howard for assistance with manuscript preparation; G.
Schoenhofer for web access for the database; members of the Farese, Vale and Walter …

We used fluorescence imaging with one nanometer accuracy (FIONA) to analyze organelle
movement by conventional kinesin and cytoplasmic dynein in a cell. We located a green …

… S3, F and G). By examining the effects of RNAi of these genes on centrosomin and γ-tubulin
localization, we suggest a molecular pathway leading to γ-tubulin localization at the spindle …

As scientists, we are at least as excited about the open questions—the things we do not
know—as the discoveries. Here, we asked 15 experts to describe the most compelling open …

In textbooks, the mitotic spindles of plants are often described separately from those of
animals. How do they differ at the molecular and mechanistic levels? In this chapter, we first …

… Sharp and G. Rogers for their comments on the manuscript and sharing their unpublished …
G. Goshima is the recipient of a post-doctoral fellowship from Japan Society for the Promotion …

Since the discovery of γ-tubulin, attention has focused on its involvement as a microtubule
nucleator at the centrosome. However, mislocalization of γ-tubulin away from the centrosome …

… In addition, we frequently observed bipolar spindles with a single g-tubulin signal at only
one pole (‘‘monastral bipolar spindle’’) near the critical expression level of Klp61F. Our …

… The lysate was clarified by centrifugation at 35,000 g for 35 min. Supernatant was incubated
with … G. Goshima is the recipient of a postdoctoral fellowship from Human Frontier Science …