… /ECAR ratio increased to a similar degree in fibroblasts, NI NPCs, and neurons compared
to PSCs, suggesting a predominance of OXPHOS over glycolysis in these cell types (Figure 2G…

High attrition rates and loss of capital plague the drug discovery process. This is particularly
evident for mitochondrial disease that typically involves neurological manifestations and is …

Leigh syndrome (LS) is a severe manifestation of mitochondrial disease in children and is
currently incurable. The lack of effective models hampers our understanding of the …

… The immunocomplexes were collected by adding 40 µl of protein G magnetic beads (Cell
Signaling), and incubated for 15 min on a rotating wheel at room temperature. Beads were …

… iPSCs derived from patient fibroblasts contained unaltered levels of the homoplasmic
mutation 8993T>G. Using somatic cell nuclear transfer (SCNT), the authors replaced the patient …

… (G) Puromycin resistant HEK293 colonies are shown as the readout of the assay. The
constructs were transfected in various combinations. HEK293 cells transfected with the mPB …

… C and SVS1C (n = 2 independent experiments), g, Representative electrophysiology traces
showing that 8 w DA cultures from SVS1C.C contained neurons showing multiple spiking …

A systematic account is given of the literature data on the mechanism of the electrophilic
halogenation of olefins. Particular attention is devoted to the experimental methods for the …

… To prepare cell-free extracts, yeast cells were harvested by centrifugation at 5000 g for 5
min, washed with a 10 mM potassium … Yeast species and strain Product yield, g/g xylose …

… In cultivation mode 2 (OTR = 5 mmol O2/(lh)), D-xylose was consumed at a significant rate
(0.46 g/(lh)), but the specific growth rate did not exceed 0.05 h–1. The oxygen supply rate in …