… Mass-spectral analyses of human enzyme preparations have given masses ranging from
54.6kDa for purified placental TrxR1 that lacks the N-terminal residues Met and Asn (G. Powis, …

… SOUTHORN MB, BS a , GARTH POWIS D. Phil. b … (10) 2 G S H + H 2 O 2 g l u t a t h i o
n e p e r o x i d a s e → G S S G + 2 H 2 O (11) 2 H 2 O 2 c a t a l a s e → O 2 + 2 H 2 O …

… Furthermore, high levels of thioredoxin-1 in the tumor appear to be associated with
decreased patient survival (J Raffel, AK Bhattacharyya & G Powis unpublished observations). …

… The cells were scraped free from the surface of the dish and centrifuged at 6000 X g for 10
mm. The supernatant was mixed with 50 pAwashed IgG2bk antiphospho tyrosine antibody …

… in nonsmoking patients, is a G to A transition (92% of mutations); a G to A transition at codon
… In NSCLC in patients who smoke, the most common KRAS mutation is a G to T transversion …

Purpose BRAF V600E mutation is seen in 5% to 8% of patients with metastatic colorectal
cancer (CRC) and is associated with poor prognosis. Vemurafenib, an oral BRAF V600 inhibitor…

Hypoxia is an inadequate oxygen supply to tissues and cells, which can restrict their function.
The hypoxic response is primarily mediated by the hypoxia-inducible transcription factors, …

The thioredoxins are ubiquitous proteins containing a conserved -Trp-Cys-Gly-Pro-Cys-Lys-
redox catalytic site. Mammalian thioredoxin family members include thioredoxin-1 (Trx1), …

… The homogenates were immediately centrifuged for 10 min at 600 × g and 4C; the … × g and
4C. The pellet was resuspended in ice-cold MSH buffer and centrifuged for 10 min at 600 × g …

Quinones are among the most frequently used drugs to treat human cancer. All of the
antitumor quinones can undergo reversible enzymatic reduction and oxidation, and form …