Hepatitis C virus RNA is 5′-capped with flavin adenine dinucleotide

…, LV Pham, C Fernandez-Antunez, G Indrisiunaite… - Nature, 2023 - nature.com
RNA viruses have evolved elaborate strategies to protect their genomes, including 5′
capping. However, until now no RNA 5′ cap has been identified for hepatitis C virus 1 , 2 (HCV), …

[PDF][PDF] How 2'-O-Methylation in mRNA Disrupts tRNA Decoding during Translation Elongation

J Choi, G Indrisiunaite, H DeMirci, KW Ieong, J Wang… - Biophysical …, 2018 - cell.com
Chemical modifications of messenger RNA (mRNA) may regulate many aspects of mRNA
processing and protein synthesis. Recently, 2'-O-methylation (Nm) of nucleotides was …

[PDF][PDF] The structural basis for release factor activation during translation termination revealed by time-resolved cryogenic electron microscopy

Z Fu, G Indrisiunaite, S Kaledhonkar, B Shah, M Sun… - Biophysical …, 2019 - cell.com
Upon encountering a stop codon in the aminoacyl site (A site) of the mRNA translating
ribosome, the ribosome recruits a class-1 release factor (RF), which induces hydrolysis of the …

Dynamics of release factor recycling during translation termination in bacteria

…, MY Pavlov, J Zhang, G Indrisiunaite… - Nucleic acids …, 2023 - academic.oup.com
In bacteria, release of newly synthesized proteins from ribosomes during translation termination
is catalyzed by class-I release factors (RFs) RF1 or RF2, reading UAA and UAG or UAA …

[HTML][HTML] On the pH dependence of class-1 RF-dependent termination of mRNA translation

G Indrisiunaite, MY Pavlov, V Heurgué-Hamard… - Journal of molecular …, 2015 - Elsevier
We have studied the pH dependence of the rate of termination of bacterial protein synthesis
catalyzed by a class-1 release factor (RF1 or RF2). We used a classical quench-flow …

N 6-Methyladenosines in mRNAs reduce the accuracy of codon reading by transfer RNAs and peptide release factors

KW Ieong, G Indrisiunaite, A Prabhakar… - Nucleic Acids …, 2021 - academic.oup.com
We used quench flow to study how N 6 -methylated adenosines (m 6 A) affect the accuracy
ratio between k cat /K m (ie association rate constant (k a ) times probability (P p ) of product …

[HTML][HTML] Accuracy of protein synthesis and its tuning by mRNA modifications

G Indrisiunaite - 2019 - diva-portal.org
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science
and Technology 1814. 47 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-513-0667-4…

[PDF][PDF] The pH dependence of peptide release from the ribosome

G Indrisiunaite - files.webb.uu.se
We have employed two different fast kinetic methods to study the pH dependence of the rate
of peptide release reaction (termination) in protein synthesis. To this end we have used a …

N6-methyladenosines in mRNA have profound effects on the accuracy of codon reading by tRNAs and peptide release factors

KW Ieong, G Indrisiunaite, M Ehrenberg - 2019 - diva-portal.org
The ribosome is a large macromolecular complex that synthesizes all proteins in the cell in
all kingdoms of life. Proteins perform many vital functions, ranging from catalysis of …

[BOOK][B] Novel Developments in Cryo‐EM of Biological Molecules: Resolution in Time and State Space

J Frank - 2023 - books.google.com
Cryo-EM, as it is currently practiced in many laboratories, is limited to the visualization of
molecules that are in thermal equilibrium at the time before freezing. A further limitation is that …