Conserved network of proteins essential for bacterial viability

JI Handford, B Ize, G Buchanan, GP Butland… - Journal of …, 2009 - Am Soc Microbiol
The yjeE, yeaZ, and ygjD genes are highly conserved in the genomes of eubacteria, and
ygjD orthologs are also found throughout the Archaea and eukaryotes. In this study, we have …

Unintended Laboratory-Driven Evolution Reveals Genetic Requirements for Biofilm Formation by Desulfovibrio vulgaris Hildenborough

…, GM Zane, VV Trotter, GP Krantz, AP Arkin, GP Butland… - MBio, 2017 - Am Soc Microbiol
Biofilms of sulfate-reducing bacteria (SRB) are of particular interest as members of this group
are culprits in corrosion of industrial metal and concrete pipelines as well as being key …

Independence of Nitrate and Nitrite Inhibition of Desulfovibrio vulgaris Hildenborough and Use of Nitrite as a Substrate for Growth

HL Korte, A Saini, VV Trotter, GP Butland… - … science & technology, 2015 - ACS Publications
Sulfate-reducing microbes, such as Desulfovibrio vulgaris Hildenborough, cause “souring”
of petroleum reservoirs through produced sulfide and precipitate heavy metals, either as …

Large-scale genetic characterization of a model sulfate-reducing bacterium

…, AM Deutschbauer, JM Chandonia, GP Butland - BioRxiv, 2021 - biorxiv.org
Sulfate-reducing bacteria (SRB) are obligate anaerobes that can couple their growth to the
reduction of sulfate. Despite the importance of SRB to global nutrient cycles and their …

[HTML][HTML] Large-scale genetic characterization of the model sulfate-reducing bacterium, Desulfovibrio vulgaris Hildenborough

…, JM Chandonia, GP Butland - Frontiers in …, 2023 - frontiersin.org
Sulfate-reducing bacteria (SRB) are obligate anaerobes that can couple their growth to the
reduction of sulfate. Despite the importance of SRB to global nutrient cycles and their …

Rapid quantification of mutant fitness in diverse bacteria by sequencing randomly bar-coded transposons

…, J He, CA Hoover, MJ Blow, J Bristow, G Butland… - MBio, 2015 - Am Soc Microbiol
Transposon mutagenesis with next-generation sequencing (TnSeq) is a powerful approach
to annotate gene function in bacteria, but existing protocols for TnSeq require laborious …

Interaction network containing conserved and essential protein complexes in Escherichia coli

G Butland, JM Peregrín-Alvarez, J Li, W Yang, X Yang… - Nature, 2005 - nature.com
… Statistical methods such as mutual information, Pearson correlation coefficient, Hamming
distance (D) and the chance co-occurrence probability distribution (P) have been developed to …

Two conserved glutamates in the bacterial nitric oxide reductase are essential for activity but not assembly of the enzyme

G Butland, S Spiro, NJ Watmough… - Journal of …, 2001 - Am Soc Microbiol
… cycA promoter to drive aerobic expression of NOR in P. denitrificans and (ii) a heterologous
… ) were introduced into NOR, and the proteins were expressed in P. denitrificans and E. coli. …

[HTML][HTML] Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins

P Hu, SC Janga, M Babu, JJ Díaz-Mejía, G Butland… - PLoS …, 2009 - journals.plos.org
… nonredundant genome dataset, filtered at 90% similarity based on the frequency of shared
orthologs (Figure 1G), with an average of 0.22 as compared with 0.48 for annotated genes (p

Global landscape of protein complexes in the yeast Saccharomyces cerevisiae

…, G Musso, P St Onge, S Ghanny, MHY Lam, G Butland… - Nature, 2006 - nature.com
Identification of protein–protein interactions often provides insight into protein function, and
many cellular processes are performed by stable protein complexes. We used tandem affinity …