Mass spectrometry (MS)-based proteomics typically employs multistep sample-preparation
workflows that are subject to sample contamination and loss. We report an in-StageTip …

Circular RNAs (circRNAs) are broadly expressed in eukaryotic cells, but their molecular
mechanism in human disease remains obscure. Here we show that circular antisense non-…

Proteins in the circulatory system mirror an individual's physiology. In daily clinical practice,
protein levels are generally determined using single-protein immunoassays. High-throughput…

Across a variety of Mendelian disorders, ∼50–75% of patients do not receive a genetic
diagnosis by exome sequencing indicating disease-causing variants in non-coding regions. …

DNMT1 is recruited by PCNA and UHRF1 to maintain DNA methylation after replication.
UHRF1 recognizes hemimethylated DNA substrates via the SRA domain, but also repressive …

Background The oral cavity is home to one of the most diverse microbial communities of the
human body and a major entry portal for pathogens. Its homeostasis is maintained by saliva, …

DNA methylation and histone modifications play a central role in the epigenetic regulation of
gene expression and cell differentiation. Recently, Np95 (also known as UHRF1 or ICBP90) …

A novel chemoenzymatic approach for simple and fast site‐specific protein labeling is reported.
Recombinant tubulin tyrosine ligase (TTL) was repurposed to attach various unnatural …

Establishment of cell polarity—or symmetry breaking—relies on local accumulation of polarity
regulators. Although simple positive feedback is sufficient to drive symmetry breaking, it is …

DNA methylation plays a central role in the epigenetic regulation of gene expression during
development and disease. Remarkably, the complex and changing patterns of genomic …